Document Detail

Community Analysis of Plant Biomass-Degrading Microorganisms from Obsidian Pool, Yellowstone National Park.
MedLine Citation:
PMID:  25319238     Owner:  NLM     Status:  Publisher    
The conversion of lignocellulosic biomass into biofuels can potentially be improved by employing robust microorganisms and enzymes that efficiently deconstruct plant polysaccharides at elevated temperatures. Many of the geothermal features of Yellowstone National Park (YNP) are surrounded by vegetation providing a source of allochthonic material to support heterotrophic microbial communities adapted to utilize plant biomass as a primary carbon and energy source. In this study, a well-known hot spring environment, Obsidian Pool (OBP), was examined for potential biomass-active microorganisms using cultivation-independent and enrichment techniques. Analysis of 33,684 archaeal and 43,784 bacterial quality-filtered 16S rRNA gene pyrosequences revealed that archaeal diversity in the main pool was higher than bacterial; however, in the vegetated area, overall bacterial diversity was significantly higher. Of notable interest was a flooded depression adjacent to OBP supporting a stand of Juncus tweedyi, a heat-tolerant rush commonly found growing near geothermal features in YNP. The microbial community from heated sediments surrounding the plants was enriched in members of the Firmicutes including potentially (hemi)cellulolytic bacteria from the genera Clostridium, Anaerobacter, Caloramator, Caldicellulosiruptor, and Thermoanaerobacter. Enrichment cultures containing model and real biomass substrates were established at a wide range of temperatures (55-85 °C). Microbial activity was observed up to 80 °C on all substrates including Avicel, xylan, switchgrass, and Populus sp. Independent of substrate, Caloramator was enriched at lower (<65 °C) temperatures while highly active cellulolytic bacteria Caldicellulosiruptor were dominant at high (>65 °C) temperatures.
Tatiana A Vishnivetskaya; Scott D Hamilton-Brehm; Mircea Podar; Jennifer J Mosher; Anthony V Palumbo; Tommy J Phelps; Martin Keller; James G Elkins
Related Documents :
23183258 - Hormonal regulation of stem cell maintenance in roots.
11971638 - Effect of insecticides on plankton and fish of ignacio ramirez reservoir (mexico): a bi...
14566558 - Secondary succession is influenced by belowground insect herbivory on a productive site.
25237348 - Hptlc analysis, antioxidant and antigout activity of indian plants.
24799708 - Variation in leaf flushing date influences autumnal senescence and next year's flushing...
23817398 - Post mortem changes in relation to different types of clothing.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-10-16
Journal Detail:
Title:  Microbial ecology     Volume:  -     ISSN:  1432-184X     ISO Abbreviation:  Microb. Ecol.     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-10-16     Completed Date:  -     Revised Date:  2014-10-17    
Medline Journal Info:
Nlm Unique ID:  7500663     Medline TA:  Microb Ecol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Measuring health-related problem solving among African Americans with multiple chronic conditions: a...
Next Document:  Two Streptomyces Species Producing Antibiotic, Antitumor, and Anti-Inflammatory Compounds Are Widesp...