| Communication among neurons. | |
| | |
MedLine Citation:
|
PMID: 22459725 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The communication among neurons is the prerequisite for the working brain. To understand the cellular, neurochemical, and structural basis of this communication, and the impacts of aging and disease on brain function, quantitative measures are necessary. This thesis evaluates several quantitative neurobiological methods with respect to possible bias and methodological issues. Stereological methods are suited for the unbiased estimation of number, length, and volumes of components of the nervous system. Stereological estimates of the total length of myelinated nerve fibers were made in white matter of post mortem brains, and the impact of aging and diseases as Schizophrenia and Alzheimer's disease were evaluated. Although stereological methods are in principle unbiased, shrinkage artifacts are difficult to account for. Positron emission tomography (PET) recordings, in conjunction with kinetic modeling, permit the quantitation of radioligand binding in brain. The novel serotonin 5-HT4 antagonist [11C]SB207145 was used as an example of the validation process for quantitative PET receptor imaging. Methods based on reference tissue as well as methods based on an arterial plasma input function were evaluated with respect to precision and accuracy. It was shown that [11C]SB207145 binding had high sensitivity to occupancy by unlabeled ligand, necessitating high specific activity in the radiosynthesis to avoid bias. The established serotonin 5-HT2A ligand [18F]altanersin was evaluated in a two-year follow-up study in elderly subjects. Application of partial volume correction of the PET data diminished the reliability of the measures, but allowed for the correct distinction between changes due to brain atrophy and receptor availability. Furthermore, a PET study of patients with Alzheimer's disease with the serotonin transporter ligand [11C]DASB showed relatively preserved serotonergic projections, despite a marked decrease in 5-HT2A receptor binding. Possible confounders are considered and the relation to the prevailing beta-amyloid hypothesis is discussed. |
| | |
Authors:
|
Lisbeth Marner |
Related Documents
:
|
22357845 - The representation of biological classes in the human brain. 14169725 - Habituation of responses to novel stimuli in monkeys with selective frontal lesions. 22892425 - Individual differences in expert motor coordination associated with white matter micros... 22828865 - Identified peptidergic neurons in the drosophila brain regulate insulin-producing cells... 7721985 - The efferent projections of the periaqueductal gray in the rat: a phaseolus vulgaris-le... 306185 - Nucleus isthmi of the frog: structure and tecto-isthmic projection. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Danish medical journal Volume: 59 ISSN: 2245-1919 ISO Abbreviation: Dan Med J Publication Date: 2012 Apr |
Date Detail:
|
Created Date: 2012-03-30 Completed Date: 2012-08-02 Revised Date: 2012-09-06 |
Medline Journal Info:
|
Nlm Unique ID: 101576205 Medline TA: Dan Med J Country: Denmark |
Other Details:
|
Languages: eng Pagination: B4427 Citation Subset: IM |
Affiliation:
|
PET and Cyclotron Unit, University Hospital of Copenhagen, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark. lisbeth@marner.dk |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent Adult Age Factors Aged Aged, 80 and over Aging / pathology, physiology* Brain / pathology, physiology* Case-Control Studies Confidence Intervals Female Humans Magnetic Resonance Imaging Male Middle Aged Nerve Fibers, Myelinated Neurons / physiology* Positron-Emission Tomography Presynaptic Terminals / physiology Schizophrenia / pathology Statistics as Topic Young Adult |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The effect of selective serotonin reuptake inhibitors in healthy first-degree relatives of patients ...
Next Document: Aspects of survival from colorectal cancer in Denmark.