Document Detail

Common lipopolysaccharide specificity: new type of antigen residing in the inner core region of S- and R-form lipopolysaccharides from different families of gram-negative bacteria.
MedLine Citation:
PMID:  6194116     Owner:  NLM     Status:  MEDLINE    
A new antigenic specificity, referred to here as common lipopolysaccharide (LPS) specificity, is described in the LPSs of gram-negative bacteria belonging to various families. The specificity is present in S- and R-form LPS but absent in Re mutants of different enterobacterial genera. By the use of purified LPS and monospecific antibodies obtained by immunoabsorption, the specificity is differentiated from the known core specificities of the genus Salmonella and the lipid A specificity by aid of the passive hemolysis and passive hemolysis inhibition test. In Salmonella minnesota R-form LPS, the specificity may be cryptic (R345, Rb2 mutant) or partly exposed in the intact molecule (R7, Rd1 mutant). The specificity is either demasked or completely exposed after mild acid hydrolysis for a short time, whereas it is destroyed after prolonged hydrolysis. Periodate oxidation, reduction, and hydrolysis under conditions that do not affect the ketosidic linkages of 2-keto-3-deoxyoctulosonic acid destroy the specificity in R4 (Rd2 mutant) LPS, but do not do so in R7 LPS. It is suggested that 2-keto-3-deoxyoctulosonic acid and a following neutral sugar are the compositional requirements for expressing the specificity.
H Brade; C Galanos
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  42     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1983 Oct 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  250-6     Citation Subset:  IM    
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MeSH Terms
Bacteroidaceae / immunology
Enterobacteriaceae / immunology
Epitopes / immunology
Gram-Negative Bacteria / immunology*
Lipid A / immunology
Lipopolysaccharides / analysis,  immunology*
Neisseriaceae / immunology
Pseudomonadaceae / immunology
Rhodospirillaceae / immunology
Vibrionaceae / immunology
Reg. No./Substance:
0/Epitopes; 0/Lipid A; 0/Lipopolysaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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