Document Detail

Common effects of acidic activators on large-scale chromatin structure and transcription.
MedLine Citation:
PMID:  15657424     Owner:  NLM     Status:  MEDLINE    
Large-scale chromatin decondensation has been observed after the targeting of certain acidic activators to heterochromatic chromatin domains. Acidic activators are often modular, with two or more separable transcriptional activation domains. Whether these smaller regions are sufficient for all functions of the activators has not been demonstrated. We adapted an inducible heterodimerization system to allow systematic dissection of the function of acidic activators, individual subdomains within these activators, and short acidic-hydrophobic peptide motifs within these subdomains. Here, we demonstrate that large-scale chromatin decondensation activity is a general property of acidic activators. Moreover, this activity maps to the same acidic activator subdomains and acidic-hydrophobic peptide motifs that are responsible for transcriptional activation. Two copies of a mutant peptide motif of VP16 (viral protein 16) possess large-scale chromatin decondensation activity but minimal transcriptional activity, and a synthetic acidic-hydrophobic peptide motif had large-scale chromatin decondensation activity comparable to the strongest full-length acidic activator but no transcriptional activity. Therefore, the general property of large-scale chromatin decondensation shared by most acidic activators is not simply a direct result of transcription per se but is most likely the result of the concerted action of coactivator proteins recruited by the activators' short acidic-hydrophobic peptide motifs.
Anne E Carpenter; Sevinci Memedula; Matthew J Plutz; Andrew S Belmont
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  25     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-19     Completed Date:  2005-03-09     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  958-68     Citation Subset:  IM    
Department of Cell and Structural Biology, B107 CLSL, 601 S. Goodwin Avenue, Urbana, IL 61801, USA.
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MeSH Terms
Amino Acid Motifs / physiology*
Amino Acid Sequence
Antibiotics, Antineoplastic / toxicity
CHO Cells
Chromatin / metabolism*
Green Fluorescent Proteins / metabolism
Herpes Simplex Virus Protein Vmw65 / metabolism*
Molecular Sequence Data
Sirolimus / toxicity
Trans-Activators / metabolism*
Transcription, Genetic / drug effects,  genetics*
Grant Support
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Chromatin; 0/Herpes Simplex Virus Protein Vmw65; 0/Trans-Activators; 147336-22-9/Green Fluorescent Proteins; 53123-88-9/Sirolimus

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