Document Detail


Common Amino Acid Sequences Deduced from Coding Exons of the Porcine FGF4 Gene in Two Breeds and Production of the Encoded Protein in Escherichia coli.
MedLine Citation:
PMID:  23291761     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Fibroblast growth factor 4 (FGF4) is considered a crucial gene in the development of mammalian embryos. Here we identified common amino acid sequences predicted from coding exons of the FGF4 gene in five pigs of two breeds, and HispFGF4, a 6× histidine-tagged porcine FGF4, was produced in Escherichia coli. HispFGF4 was purified efficiently from the supernatant of cell lysate by heparin column chromatography. In a porcine embryonic fibroblast cell line, HispFGF4 showed significant mitogenic activities at concentrations as low as 0.001 nM (p<0.01). To the best of our knowledge, this is the first report describing the complete nucleotide sequence of coding exons for the porcine FGF4 protein in two breeds, together with the production of a recombinant, bioactive porcine FGF4 derivative.
Authors:
Saiko Sugawara; Toshihiko Ito; Hitoshi Suzuki; Toshikiyo Takahashi; Jun-Ichi Konishi; Masaki Kobayashi; Sho Sato; Yuki Mori; Kano Kasuga; Ikuo Kojima; Tomokazu Fukuda; Masayuki Kobayashi
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  -     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Graduate School of Bioresource Sciences, Akita Prefectural University.
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