Document Detail


Commitment of apolipoprotein B RNA to the splicing pathway regulates cytidine-to-uridine editing-site utilization.
MedLine Citation:
PMID:  11672445     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A tripartite motif located in the centre of the 7.5 kb exon 26 of apolipoprotein B (apoB) mRNA directs editosome assembly and site-specific cytidine-to-uridine editing at nucleotide 6666. apoB mRNA editing is a post-transcriptional event, occurring primarily at the time exon 26 is spliced or at a time after splicing, but before nuclear export. We show, through reporter RNA constructs, that RNA splice sites suppress editing of precursor RNAs when placed proximal or distal to the editing site. Processed RNAs were edited more efficiently than precursor RNAs. Mutation of both the splice donor and acceptor sites was necessary for RNAs to be edited efficiently. The results suggested that commitment of pre-mRNA to the splicing and/or nuclear-export pathways may play a role in regulating editing-site utilization. The HIV-1 Rev-Rev response element ('RRE') interaction was utilized to uncouple the commitment of precursor RNAs to the spliceosome assembly pathway and associated nuclear-export pathway. Under these conditions, unspliced reporter RNAs were edited efficiently. We propose that pre-mRNA passage through the temporal or spatial restriction point where they become committed to spliceosome assembly contributes regulatory information for subsequent editosome activity.
Authors:
M P Sowden; H C Smith
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Biochemical journal     Volume:  359     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-10-23     Completed Date:  2001-12-13     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  697-705     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apolipoproteins B / genetics*
Genes, env / genetics
Humans
Introns / genetics
RNA Editing / genetics*
RNA Splicing / genetics*
Rats
Spliceosomes / metabolism
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
DK43739/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Apolipoproteins B
Comments/Corrections

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