Document Detail


Commercial baker's yeast stability as affected by intracellular content of trehalose, dehydration procedure and the physical properties of external matrices.
MedLine Citation:
PMID:  11092635     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of vacuum-drying and freeze-drying on the cell viability of a commercial baker's yeast, Saccharomyces cerevisiae, strain with different endogenous contents of trehalose were analyzed. An osmotolerant Zygosaccharomyces rouxii strain was used for comparative purposes. Higher viability values were observed in cells after vacuum-drying than after freeze-drying. Internal concentrations of trehalose in the range 10-20% protected cells in both dehydration processes. Endogenous trehalose concentrations did not affect the water sorption isotherm nor the Tg values. The effect of external matrices of trehalose and maltodextrin was also studied. The addition of external trehalose improved the survival of S. cerevisiae cells containing 5% internal trehalose during dehydration. Maltodextrin (1.8 kDa) failed to protect vacuum-dried samples at 40 degrees C. The major reduction in the viability during the freeze-drying process of the sensitive yeast cells studied was attributed to the freezing step. The suggested protective mechanisms for each particular system are vitrification and the specific interactions of trehalose with membranes and/or proteins. The failure of maltodextrins to protect cells was attributed to the fact that none of the suggested mechanisms of protection could operate in these systems.
Authors:
P Cerrutti; M Segovia de Huergo; M Galvagno; C Schebor; M del Pilar Buera
Related Documents :
22079695 - Human hypoblast formation is not dependent on fgf signalling.
17388245 - The use of phosphate buffered saline for the recovery of cells and spermatozoa from swabs.
23474115 - Sorafenib enhances radiation-induced apoptosis in hepatocellular carcinoma by inhibitin...
12522505 - Relationship between the changes in cellular volume of fish spermatozoa and their cryor...
18463485 - Pseudomonas aeruginosa quorum-sensing signal molecules induce il-8 production by human ...
16387775 - Filamin-a and rheological properties of cultured melanoma cells.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  54     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2001-02-23     Completed Date:  2001-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  575-80     Citation Subset:  IM    
Affiliation:
Departamento de Ingeniería Química, Facultad de Ingenieria, Universidad de Buenos Aires, Ciudad Universitaria, Argentina.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Freeze Drying
Saccharomyces cerevisiae / chemistry,  physiology*
Trehalose / analysis*
Chemical
Reg. No./Substance:
99-20-7/Trehalose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Fibrobacter succinogenes S85 ferments ball-milled cellulose as fast as cellobiose until cellulose su...
Next Document:  Zinc biosorption by a zinc-resistant bacterium, Brevibacterium sp. strain HZM-1.