Document Detail


Combined transcript and metabolite profiling of Arabidopsis leaves reveals fundamental effects of the thiol-disulfide status on plant metabolism.
MedLine Citation:
PMID:  16648214     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we used gas chromatography-mass spectrometry analysis in combination with flux analysis and the Affymetrix ATH1 GeneChip to survey the metabolome and transcriptome of Arabidopsis (Arabidopsis thaliana) leaves in response to manipulation of the thiol-disulfide status. Feeding low concentrations of the sulfhydryl reagent dithiothreitol for 1 h at the end of the dark period led to posttranslational redox activation of ADP-glucose pyrophosphorylase and major alterations in leaf carbon partitioning, including an increased flux into major respiratory pathways, starch, cell wall, and amino acid synthesis, and a reduced flux to sucrose. This was accompanied by a decrease in the levels of hexose phosphates, while metabolites in the second half of the tricarboxylic acid cycle and various amino acids increased, indicating a stimulation of anaplerotic fluxes reliant on alpha-ketoglutarate. There was also an increase in shikimate as a precursor of secondary plant products and marked changes in the levels of the minor sugars involved in ascorbate synthesis and cell wall metabolism. Transcript profiling revealed a relatively small number of changes in the levels of transcripts coding for components of redox regulation, transport processes, and cell wall, protein, and amino acid metabolism, while there were no major alterations in transcript levels coding for enzymes involved in central metabolic pathways. These results provide a global picture of the effect of redox and reveal the utility of transcript and metabolite profiling as systemic strategies to uncover the occurrence of redox modulation in vivo.
Authors:
Anna Kolbe; Sandra N Oliver; Alisdair R Fernie; Mark Stitt; Joost T van Dongen; Peter Geigenberger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-28
Journal Detail:
Title:  Plant physiology     Volume:  141     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-08     Completed Date:  2006-08-28     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  412-22     Citation Subset:  IM    
Affiliation:
Max-Planck Institute of Molecular Plant Physiology, 14476 Golm-Potsdam, Germany.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / enzymology,  genetics*,  metabolism
Dithiothreitol / metabolism*
Oxidation-Reduction
Plant Leaves / enzymology,  metabolism*
Protein Processing, Post-Translational
RNA, Messenger / genetics*
Chemical
Reg. No./Substance:
0/RNA, Messenger; 3483-12-3/Dithiothreitol
Comments/Corrections

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