Document Detail


Combined time-lapse cinematography and immuno-electron microscopy.
MedLine Citation:
PMID:  2184699     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method was developed to record interactions between mobile non-adherent immunocytes by time-lapse cinematography and then to study the same cells by immuno-electron microscopy, using monoclonal antibodies against surface components. For this purpose a modified stage was designed to fit an inverted microscope. The attachment included a device to cool the culture chamber with N2 gas, a micro-injector for monoclonal antibody and immuno-gold treatment, and two pairs of washing needles to change the medium without disturbance. The technique was first employed to study the formation of aggregates around the antigen-presenting cells in cultures containing cells from hyper-immunized animals. Recently peripheral blood cells from normal subjects and patients with immune deficiency syndromes were stimulated with pokeweed mitogen, cluster formation was recorded, and the cells were processed for immuno-electron microscopy.
Authors:
B M Balfour; T Goscicka; J L MacKenzie; A Gautam; M Tate; J Clark
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Anatomical record     Volume:  226     ISSN:  0003-276X     ISO Abbreviation:  Anat. Rec.     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-05-31     Completed Date:  1990-05-31     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  0370540     Medline TA:  Anat Rec     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  509-14     Citation Subset:  IM    
Affiliation:
Clinical Research Centre, Harrow, Middlesex, England.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal
Antigen-Presenting Cells / immunology,  physiology,  ultrastructure
Cell Communication / immunology,  physiology
Immunologic Techniques*
Lymphocytes / immunology,  physiology,  ultrastructure
Microscopy, Electron / methods*
Motion Pictures as Topic*
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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