Document Detail

Combined serum bactericidal and phagocytic activities of defibrinated human blood against Serratia marcescens. Use of phenylbutazone to selectively block phagocytic killing activity and of Group A (phage tail) bacteriocins to kill extraphagocytic bacteria.
MedLine Citation:
PMID:  6754279     Owner:  NLM     Status:  MEDLINE    
Fresh, defibrinated human blood (65 vol%) from normal adults reduced cell inocula of Serratia marcescens, comprising 'delayed serum-sensitive', 'pseudo-serum-resistant', and 'non-serum-sensitive' human serum susceptibility categories, by up to approximately 3 log10 units, provided cell inocula did not significantly exceed 10(5) colony-forming units/ml at 0 time. Phenylbutazone (2 mg/ml) antagonized neither serum bactericidal activity nor the biological activity of group A (phage tail) bacteriocins bA+ 16 and bA+ 18. These bacteriocins were suitable for killing extraphagocytic S. marcescens cells, since they were not internalized by peripheral blood leukocytes. Phenylbutazone at 2 mg/ml failed to interfere with ingestion of S. marcescens by leukocytes; however, this drug inhibited intraphagocytic killing of ingested S. marcescens bacteria. Pilot experiments, utilizing this latter system, disclosed that rifampin effectively killed intraphagolysosomal bacteria.
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemotherapy     Volume:  28     ISSN:  0009-3157     ISO Abbreviation:  Chemotherapy     Publication Date:  1982  
Date Detail:
Created Date:  1983-01-07     Completed Date:  1983-01-07     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0144731     Medline TA:  Chemotherapy     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  355-62     Citation Subset:  IM    
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MeSH Terms
Bacteriocins / pharmacology*
Blood Bactericidal Activity*
Phagocytosis / drug effects*
Phenylbutazone / pharmacology*
Serratia marcescens* / drug effects
Reg. No./Substance:
0/Bacteriocins; 50-33-9/Phenylbutazone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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