Document Detail

Combined reflection and transmission microscope for telemedicine applications in field settings.
MedLine Citation:
PMID:  21709875     Owner:  NLM     Status:  MEDLINE    
We demonstrate a field-portable upright and inverted microscope that can image specimens in both reflection and transmission modes. This compact and cost-effective dual-mode microscope weighs only ∼135 grams (<4.8 ounces) and utilizes a simple light emitting diode (LED) to illuminate the sample of interest using a beam-splitter cube that is positioned above the object plane. This LED illumination is then partially reflected from the sample to be collected by two lenses, creating a reflection image of the specimen onto an opto-electronic sensor-array that is positioned above the beam-splitter cube. In addition to this, the illumination beam is also partially transmitted through the same specimen, which then casts lensfree in-line holograms of the same objects onto a second opto-electronic sensor-array that is positioned underneath the beam-splitter cube. By rapid digital reconstruction of the acquired lensfree holograms, transmission images (both phase and amplitude) of the same specimen are also created. We tested the performance of this field-portable microscope by imaging various micro-particles, blood smears as well as a histopathology slide corresponding to skin tissue. Being compact, light-weight and cost-effective, this combined reflection and transmission microscope might especially be useful for telemedicine applications in resource limited settings.
Gabriel Biener; Alon Greenbaum; Serhan O Isikman; Kelvin Lee; Derek Tseng; Aydogan Ozcan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-06-27
Journal Detail:
Title:  Lab on a chip     Volume:  11     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-07-27     Completed Date:  2011-12-13     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  2738-43     Citation Subset:  IM    
Copyright Information:
This journal is © The Royal Society of Chemistry 2011
UCLA Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA.
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MeSH Terms
Equipment Design
Metals / chemistry
Microscopy / instrumentation,  methods*
Oxides / chemistry
Grant Support
DP2 OD006427-01/OD/NIH HHS; DP2OD006427/OD/NIH HHS; R21 EB009222-02/EB/NIBIB NIH HHS
Reg. No./Substance:
0/Metals; 0/Oxides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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