| Combined off-resonance imaging and T2 relaxation in the rotating frame for positive contrast MR imaging of infection in a murine burn model. | |
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MedLine Citation:
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PMID: 21031524 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To develop novel magnetic resonance (MR) imaging methods to monitor accumulation of macrophages in inflammation and infection. Positive-contrast MR imaging provides an alternative to negative-contrast MRI, exploiting the chemical shift induced by ultra-small superparamagnetic iron-oxide (USPIO) nanoparticles to nearby water molecules. We introduce a novel combination of off-resonance (ORI) positive-contrast MRI and T(2ρ) relaxation in the rotating frame (ORI-T(2ρ)) for positive-contrast MR imaging of USPIO. MATERIALS AND METHODS: We tested ORI-T(2ρ) in phantoms and imaged in vivo the accumulation of USPIO-labeled macrophages at the infection site in a mouse model of burn trauma and infection with Pseudomonas aeruginosa (PA). PA infection is clinically important. The USPIO nanoparticles were injected directly in the animals in solution, and macrophage labeling occurred in vivo in the animal model. RESULTS: We observed a significant difference between ORI-T(2ρ) and ORI, which leads us to suggest that ORI-T(2ρ) is more sensitive in detecting USPIO signal. To this end, the ORI-T(2ρ) positive contrast method may prove to be of higher utility in future research. CONCLUSION: Our results may have direct implications in the longitudinal monitoring of infection, and open perspectives for testing novel anti-infective compounds. |
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Authors:
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Ovidiu C Andronesi; Dionyssios Mintzopoulos; Valeria Righi; Nikolaos Psychogios; Meenu Kesarwani; Jianxin He; Shingo Yasuhara; George Dai; Laurence G Rahme; Aria A Tzika |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of magnetic resonance imaging : JMRI Volume: 32 ISSN: 1522-2586 ISO Abbreviation: J Magn Reson Imaging Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-29 Completed Date: 2011-02-14 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 9105850 Medline TA: J Magn Reson Imaging Country: United States |
Other Details:
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Languages: eng Pagination: 1172-83 Citation Subset: IM |
Copyright Information:
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© 2010 Wiley-Liss, Inc. |
Affiliation:
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Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Burns / complications*, microbiology Contrast Media* Dextrans / diagnostic use* Macrophages / pathology* Magnetic Resonance Imaging / methods* Magnetite Nanoparticles / diagnostic use* Mice Mice, Inbred Strains Phantoms, Imaging Pseudomonas Infections / diagnosis, pathology Wound Infection / diagnosis, etiology, pathology* |
| Grant Support | |
ID/Acronym/Agency:
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R01AI063433/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media; 0/Magnetite Nanoparticles; 0/ferumoxtran-10; 9004-54-0/Dextrans |
| Comments/Corrections | |
Erratum In:
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J Magn Reson Imaging. 2011 Jan;33(1):256 Note: Righi, Valeria [added] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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