Document Detail


Combined non-radioactive detection of peptide hormones and their mRNA's in endocrine cells.
MedLine Citation:
PMID:  1660859     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Non-radioactive in situ hybridization employing detection of biotin-labeled probes by an alkaline phosphatase-based procedure has proven useful for demonstrating a wide variety of mRNA species. With certain developers, the alkaline phosphatase reaction product is both light microscopically visible and fluorescent. We have exploited this to perform simple double-stainings for mRNA's and their corresponding peptide products in human insulin and gastrin cells and in rat ACTH, MSH and gastrin cells. Such stainings show that nearly all of these cells simultaneously contain both the peptide hormone and its corresponding mRNA. Human gastrin cells show a differentiated localization of gastrin mRNA and gastrin. Thus, while gastrin immunofluorescence predominates in secretory granules present in the basolateral region of the cells, gastrin mRNA is virtually restricted to the supranuclear region of the cells. Here it may be preferentially associated with granular endoplasmic reticulum. The strict subcellular localization of gastrin mRNA differs from that of general polyadenylated RNA in the G cells and raises questions whether specific transport routes or sites of accumulation for defined mRNA species exist.
Authors:
L I Larsson; D M Hougaard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histochemistry     Volume:  96     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1991  
Date Detail:
Created Date:  1992-01-23     Completed Date:  1992-01-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  375-80     Citation Subset:  IM    
Affiliation:
Department of Molecular Cell Biology, State Serum Institute, Copenhagen, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Adrenocorticotropic Hormone / analysis*,  genetics
Alkaline Phosphatase / metabolism
Animals
Base Sequence
Biotin / chemistry,  metabolism
DNA Probes / genetics,  metabolism
Fluorescent Antibody Technique
Gastric Mucosa / chemistry,  cytology
Gastrins / analysis*,  genetics
Humans
Immunohistochemistry / methods*
Insulin / analysis*,  genetics
Islets of Langerhans / chemistry
Male
Melanocyte-Stimulating Hormones / analysis*,  genetics
Molecular Sequence Data
Nucleic Acid Hybridization
Pancreas / chemistry,  cytology
RNA, Messenger / analysis
Rats
Rats, Inbred Strains
Chemical
Reg. No./Substance:
0/DNA Probes; 0/Gastrins; 0/RNA, Messenger; 11061-68-0/Insulin; 58-85-5/Biotin; 9002-60-2/Adrenocorticotropic Hormone; 9002-79-3/Melanocyte-Stimulating Hormones; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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