| Combined microspectrophotometry and automated quantitative autoradiography applied to the analysis of the plant cell cycle. | |
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MedLine Citation:
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PMID: 393709 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two methods, which relate grain number to cell cycle phase in Feulgen-stained autoradiographic preparations, have been developed and compared. Both methods automate grain number estimations, one by taking integrated absorbance measurements at different wavelengths, the other by measuring absorption at a single wavelength before and after chemical removal of silver grains. With tritium-labelled tobacco mosaic virus as a probe, a quantitative analysis has been made of the binding of virus particles to plant protoplasts in different compartments of the DNA replication and partition cycle. The preliminary results indicate that the quantity of virus bound by protoplasts is related to their cell cycle phase. Whilst in this case, the methods have been used with plant cells, both techniques are equally applicable to animal cells. |
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Authors:
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A R Gould |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of cell science Volume: 39 ISSN: 0021-9533 ISO Abbreviation: J. Cell. Sci. Publication Date: 1979 Oct |
Date Detail:
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Created Date: 1980-04-17 Completed Date: 1980-04-17 Revised Date: 2001-11-28 |
Medline Journal Info:
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Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 235-45 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Autoradiography Cell Cycle* Cytological Techniques* DNA / analysis Interphase* Plants / analysis, cytology* Plants, Toxic Protoplasts / microbiology Spectrophotometry Tobacco / cytology, microbiology Tobacco Mosaic Virus / metabolism |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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