| Combined inhibition of aromatase activity and dihydrotestosterone supplementation attenuates renal injury in male streptozotocin (STZ)-induced diabetic rats. | |
| | |
MedLine Citation:
|
PMID: 22301628 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Our previous studies showed that streptozotocin (STZ)-induced diabetic male rats have increased estradiol and decreased testosterone levels that correlate with renal injury. We further showed that either supplementing dihydrotestosterone (DHT) or inhibiting estradiol biosynthesis in these diabetic rats was only partially renoprotective. The aim of this study was to test the hypothesis that the combined therapy of DHT supplementation and inhibition of estradiol synthesis would afford better renoprotection than either treatment alone. The study was performed in 12 week old male non-diabetic (ND), STZ-induced diabetic (D), and STZ-induced diabetic rats that received the combined therapy of 0.75mg/day of DHT along with 0.15mg/kg/day of an aromatase inhibitor, anastrozole (Dta) for 12 weeks. Treatment with the combined therapy resulted in attenuation of albuminuria by 84%, glomerulosclerosis by 55% and tubulointerstitial fibrosis by 62%. In addition, the combined treatment decreased the density of renal cortical CD68-positive cells by 70% and decreased protein expression of transforming growth factor-beta protein expression by 60%, collagen type IV by 65%, tumor necrosis factor-alpha by 55% and interleukin-6 by 60%. We conclude that the combined treatment of DHT and blocking aromatase activity in diabetic male STZ-induced diabetic rats provides superior treatment than either treatment alone in the prevention of diabetic renal disease. |
| | |
Authors:
|
Michaele B Manigrasso; R Taylor Sawyer; Zachary M Hutchens; Elizabeth R Flynn; Christine Maric-Bilkan |
Related Documents
:
|
3554198 - Immunotherapy in type i diabetes. approaches to prevention and treatment. 18718668 - Changes in the internal structure of the human crystalline lens with diabetes mellitus ... 18771908 - Desaturase activities and metabolic control in type 2 diabetes. 20309918 - Type 2 diabetes and cancer: what is the connection? 8763398 - Adrenergic, insulin, and work interactions with adenosine's effects on in situ myocardi... 10415018 - Il-18 inhibits diabetes development in nonobese diabetic mice by counterregulation of t... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-2-1 |
Journal Detail:
|
Title: American journal of physiology. Renal physiology Volume: - ISSN: 1522-1466 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-2-3 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100901990 Medline TA: Am J Physiol Renal Physiol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
1University of Mississippi Medical Center. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Role of Cyclooxygenase-1-Mediated Prostacyclin Synthesis in Endothelium-Dependent Vasoconstrictor Ac...
Next Document: Carl W. Gottschalk Distinguished Lecturer of the American Physiological Society Renal Section.