Document Detail

Combined infrared multiphoton dissociation and electron-capture dissociation using co-linear and overlapping beams in Fourier transform ion cyclotron resonance mass spectrometry.
MedLine Citation:
PMID:  16705647     Owner:  NLM     Status:  MEDLINE    
A novel set-up for Fourier transform ion cyclotron resonance mass spectrometry (FTICR) is reported for simultaneous infrared multiphoton dissociation (IRMPD) and electron-capture dissociation (ECD). An unmodified electron gun ensures complete, on-axis overlap between the electron and the photon beams. The instrumentation, design and implementation of this novel approach are described. In this configuration the IR beam is directed into the ICR cell using a pneumatically actuated mirror inserted into the ion-optical path. Concept validation was made using different combinations of IRMPD and ECD irradiation events on two standard peptides. The ability to perform efficient IRMPD, ECD and especially simultaneous IRMPD and ECD using lower irradiation times is demonstrated. The increase in primary sequence coverage, with the combined IRMPD and ECD set-up, also increases the confidence in peptide and protein assignments.
Romulus Mihalca; Yuri E M van der Burgt; Liam A McDonnell; Marc Duursma; Iliya Cerjak; Albert J R Heck; Ron M A Heeren
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  20     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2006  
Date Detail:
Created Date:  2006-05-29     Completed Date:  2007-07-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  1838-44     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2006 John Wiley & Sons, Ltd.
FOM Institute for Atomic and Molecular Physics (AMOLF), Kruislaan 407, 1098 SJ Amsterdam, The Netherlands.
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MeSH Terms
Amino Acid Sequence
Melitten / chemistry
Molecular Sequence Data
Proteomics / instrumentation*,  methods*
Spectroscopy, Fourier Transform Infrared / instrumentation*,  methods*
Substance P / chemistry
Time Factors
Reg. No./Substance:
20449-79-0/Melitten; 33507-63-0/Substance P

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