| Combined effects of TNF-α, IL-1β, and HIF-1α on MMP-2 production in ACL fibroblasts under mechanical stretch: An in vitro study. | |
| | |
MedLine Citation:
|
PMID: 21344498 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The dynamics between inflammatory factors, mechanical stress, and healing factors, in an intra-articular joint, are very complex after injury. Injury to intra-articular tissue [anterior cruciate ligament (ACL), synovium] results in hypoxia, accumulation of various pro-inflammatory factors, cytokines, and metalloproteases. Although the presence of increased amounts of matrix-metalloproteinases (MMP) in the joint fluid after knee injury is considered the key factor for ACL poor healing ability; however, the exact role of collective participants of the joint fluid on MMP-2 activity and production has not been fully studied yet. To investigate the combined effects of mechanical injury, inflammation and hypoxia induced factor-1α (HIF-1α) on induction of MMP-2; we mimicked the microenvironment of joint cavity after ACL injury. The results show that TNF-α and IL-1β elevate the activity of MMP-2 in a dose- and time-dependent manner. In addition, mechanical stretch further enhances the MMP-2 protein levels with TNF-α, IL-1β, and their mixture. CoCl(2) -induced HIF-1α (100 and 500 µM) also increases the levels and activity of MMP-2. Mechanical stretch has a strong additional effect on MMP-2 production with HIF-1α. Our results conclude that mechanical injury, HIF-1α and inflammatory factors collectively induce increased MMP-2 production in ACL fibroblasts, which was inhibited by NF-κB pathway inhibitor (Bay-11-7082). © 2011 Orthpaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res. |
| | |
Authors:
|
Yequan Wang; Zhenyu Tang; Ruyue Xue; Gurinder K Singh; Kunning Shi; Yonggang Lv; Li Yang |
Related Documents
:
|
17488198 - Aging impairs igf-i receptor activation and induces skeletal resistance to igf-i. 2416458 - A significant part of macrophage-derived growth factor consists of at least two forms o... 9823298 - The phosphoinositol phosphatase activity of pten mediates a serum-sensitive g1 growth a... 9037698 - Regulators of adipocyte precursor cells. 21547728 - Measurement of fluid secretion from intact airway submucosal glands. 9076698 - Does human toxoplasmosis involve an imbalance in t1/t2 cytokines? |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-2-22 |
Journal Detail:
|
Title: Journal of orthopaedic research : official publication of the Orthopaedic Research Society Volume: - ISSN: 1554-527X ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
|
Created Date: 2011-2-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8404726 Medline TA: J Orthop Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Orthpaedic Research Society. |
Affiliation:
|
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, PR China; "111" Biomechanics and Tissue Repair Laboratory, Bioengineering College, Chongqing University, Chongqing 400044, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of low-magnitude, high-frequency vibration on osteogenic differentiation of rat mesenchymal s...
Next Document: Effect of intermittent administration of adiponectin on bone regeneration following mandibular osteo...