Document Detail

Combined effects of CXCL8 and CXCR2 gene polymorphisms on susceptibility to systemic sclerosis.
MedLine Citation:
PMID:  22763041     Owner:  NLM     Status:  MEDLINE    
METHODS: One fifty one patients with SSc and 147 healthy bone marrow donors were enrolled in a case-control study. Blood was collected for DNA extraction; typing of CXCL8 (-251) T/A and CXCR2 (+1208) T/C genes was made by polymerase chain reaction with sequence specific primers (PCR-SSP), followed by agarose gel electrophoresis.
RESULTS: The CXCR2-TC genotype was significantly less frequent in patients (23.8% versus 55.1% in controls; P<0.001, OR=0.26, 95%CI=0.15-0.43), whereas the CXCR2-CC genotype was significantly more frequent (44.4% versus 22.4% in controls; P<0.001, OR=2.76, 95%CI, 1.62-4.72). When CXCR2 and CXCL8 combinations were analyzed, the presence of CXCR2 T in the absence of CXCL8 A (CXCR2 T+/CXCL8 A-) was more frequent in patients than in controls (34.5% versus 3.5%; P<0.001, OR=14.50, 95%CI=5.04-41.40). However, CXCR2 TT and CXCL8 A were significantly more common in controls (100%) than in patients (58.3%) (P<0.001). Likewise, the presence of CXCR2 TC and CXCL8 A was more frequent in controls (95.1%) than in patients (75%) (P=0.004). Furthermore, the CXCR2-CC genotype in CXCL8 A was more frequent in patients (59.7% versus 0% in controls; P<0.001, adjusted OR=98.67, 95%CI=6.04-1610.8). In patients, a high frequency was observed in combination with the CXCL8 TA and AA genotypes (P<0.001; OR=28.92), whereas in controls, there was a high frequency of combination with CXCL8 T (P<0.001; OR=0.03) and TT (P<0.001; OR=0.01).
CONCLUSIONS: These findings suggest a protective role of CXCL8 (-251) A in the CXCR2 (+1208) TT and TC genotypes and an increased risk of CXCL8 (-251) A in association with the CXCR2 (+1208) CC genotype in SSc patients.
Patricia Hartstein Salim; Mariana Jobim; Markus Bredemeier; José Arthur Bogo Chies; João Carlos Tavares Brenol; Luiz Fernando Jobim; Ricardo Machado Xavier
Publication Detail:
Type:  Journal Article     Date:  2012-07-03
Journal Detail:
Title:  Cytokine     Volume:  60     ISSN:  1096-0023     ISO Abbreviation:  Cytokine     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-01     Completed Date:  2013-02-19     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  9005353     Medline TA:  Cytokine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  473-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
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MeSH Terms
Case-Control Studies
Gene Frequency / genetics
Genetic Predisposition to Disease*
Interleukin-8 / genetics*
Polymorphism, Single Nucleotide / genetics*
Receptors, Interleukin-8B / genetics*
Scleroderma, Systemic / genetics*
Reg. No./Substance:
0/IL8 protein, human; 0/Interleukin-8; 0/Receptors, Interleukin-8B

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