Document Detail

Combined effect of dopamine and MPP+ on membrane permeability in mitochondria and cell viability in PC12 cells.
MedLine Citation:
PMID:  12620283     Owner:  NLM     Status:  MEDLINE    
The present study examined the combined effect of dopamine and 1-methyl-4-phenylpyridinium (MPP(+)) on the membrane permeability in isolated brain mitochondria and on cell viability in PC12 cells. MPP(+) increased effect of dopamine against the swelling, membrane potential, and Ca(2+) transport in isolated mitochondria, which was not inhibited by the addition of antioxidant enzymes (SOD and catalase). Dopamine or MPP(+) caused the decrease in transmembrane potential, increase in reactive oxygen species, depletion of GSH, and cell death in PC12 cells. Antioxidant enzymes reduced each effect of dopamine and MPP(+) against PC12 cells. Co-addition of dopamine and MPP(+) caused the decrease in the transmembrane potential and increase in the formation of reactive oxygen species in PC12 cells, in which they showed an additive effect. Dopamine plus MPP(+)-induced the depletion of GSH and cell death in PC12 cells were not decreased by the addition of antioxidant enzymes, rutin, diethylstilbestrol, and ascorbate. Melanin caused a cell viability loss in PC12 cells. The N-acetylcysteine, N-phenylthiourea, and 5-hydroxyindole decreased the cell death and the formation of dopamine quinone and melanin induced by co-addition of dopamine and MPP(+), whereas deprenyl and chlorgyline did not show an inhibitory effect. The results suggest that co-addition of dopamine and MPP(+) shows an enhancing effect on the change in mitochondrial membrane permeability and cell death, which may be accomplished by toxic quinone and melanin derived from the MPP(+)-stimulated dopamine oxidation.
Chung Soo Lee; Eun Hyang Song; Se Young Park; Eun Sook Han
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemistry international     Volume:  43     ISSN:  0197-0186     ISO Abbreviation:  Neurochem. Int.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-03-06     Completed Date:  2003-06-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8006959     Medline TA:  Neurochem Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  147-54     Citation Subset:  IM    
Department of Pharmacology, College of Medicine, Chung-Ang University, 156-756, Seoul, South Korea.
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MeSH Terms
1-Methyl-4-phenylpyridinium / pharmacology*
Calcium / metabolism
Catalase / metabolism,  pharmacology
Cell Membrane Permeability / drug effects,  physiology*
Cell Survival / drug effects*,  physiology
Dopamine / analogs & derivatives*,  metabolism,  pharmacokinetics,  pharmacology*
Glutathione / metabolism
Intracellular Membranes / drug effects*,  physiology
Melanins / metabolism
Membrane Potentials / drug effects
Mitochondria / drug effects*,  physiology
Mitochondrial Swelling / drug effects
PC12 Cells
Superoxide Dismutase / metabolism,  pharmacology
Reg. No./Substance:
0/Melanins; 48134-75-4/1-Methyl-4-phenylpyridinium; 50673-96-6/dopamine quinone; 70-18-8/Glutathione; 7440-70-2/Calcium; EC; EC Dismutase

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