Document Detail

Combined deficiency of vitamins E and C causes paralysis and death in guinea pigs.
MedLine Citation:
PMID:  12791628     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: On the basis of in vitro studies, the antioxidant nutrients vitamins E and C are postulated to interact in vivo. OBJECTIVE: We developed a guinea pig model to evaluate the combined deficiency of vitamins E and C in vivo. DESIGN: Weanling guinea pigs were fed a control diet or a vitamin E-deficient diet for 14 d, after which one-half of each group had vitamin C removed from their diet, thus creating 4 diet groups. Some animals were observed for clinical signs. Others were killed for evaluation. RESULTS: Of 21 guinea pigs that were observed after being fed the diet deficient in both vitamins, 8 died 9 +/- 2 d (x +/- SD) after starting the diet. Eight additional guinea pigs developed a characteristic syndrome at 11 +/- 3 d. First, they became paralyzed in the hind limbs. Within a few hours, the paralysis progressed to include all 4 limbs and caused difficulty in breathing, which would have caused death had the animals not been euthanized. Histopathologic evaluation did not identify a lesion in the muscles or nervous system that could account for the paralysis. Biochemical measurements confirmed the deficiencies and indicated that the double deficiency caused lipid peroxidation in the central nervous system. CONCLUSIONS: A distinct clinical syndrome of combined vitamin E and vitamin C deficiency occurs in guinea pigs. This syndrome indicates that these antioxidant vitamins are related in vivo. We speculate that acute oxidative injury in the central nervous system underlies the clinical syndrome.
Kristina E Hill; Thomas J Montine; Amy K Motley; Xia Li; James M May; Raymond F Burk
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of clinical nutrition     Volume:  77     ISSN:  0002-9165     ISO Abbreviation:  Am. J. Clin. Nutr.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-06-06     Completed Date:  2003-07-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376027     Medline TA:  Am J Clin Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1484-8     Citation Subset:  AIM; IM    
Division of Gastroenterology, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN, USA.
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MeSH Terms
Ascorbic Acid / blood
Ascorbic Acid Deficiency / complications*
Brain / pathology
Disease Models, Animal*
Guinea Pigs
Osmolar Concentration
Paralysis / etiology*,  mortality,  pathology
Sciatic Nerve / pathology
Spinal Cord / pathology
Vitamin E Deficiency / complications*
alpha-Tocopherol / blood
Grant Support
Reg. No./Substance:
50-81-7/Ascorbic Acid; 59-02-9/alpha-Tocopherol

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