| Combined contrast-enhanced ultrasound and rt-PA treatment is safe and improves impaired microcirculation after reperfusion of middle cerebral artery occlusion. | |
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MedLine Citation:
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PMID: 20531462 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In monitoring of recanalization and in sonothrombolysis, contrast-enhanced ultrasound (CEUS) is applied in extended time protocols. As extended use may increase the probability of unwanted effects, careful safety evaluation is required. We investigated the safety profile and beneficial effects of CEUS in a reperfusion model. Wistar rats were subjected to filament occlusion of the right middle cerebral artery (MCA). Reperfusion was established after 90 minutes, followed by recombinant tissue-type plasminogen activator (rt-PA) treatment and randomization to additional CEUS (contrast agent: SonoVue; 60 minutes). Blinded outcome evaluation consisted of magnetic resonance imaging (MRI), neurologic assessment, and histology and, in separate experiments, quantitative 3D nano-computed tomography (CT) angiography (900 nm(3) voxel size). Nano-CT revealed severely compromised microcirculation in untreated animals after MCA reperfusion. The rt-PA partially improved hemispheric perfusion. Impairment was completely reversed in animals receiving rt-PA and CEUS. This combination was more effective than treatment with either CEUS without rt-PA or rt-PA and ultrasound or ultrasound alone. In MRI experiments, CEUS and rt-PA treatment resulted in a significantly reduced ischemic lesion volume and edema formation. No unwanted effects were detected on MRI, histology, and intracranial temperature assessment. This study shows that CEUS and rt-PA is safe in the situation of reperfusion and displays beneficial effects on the level of the microvasculature. |
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Authors:
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Max Nedelmann; Nouha Ritschel; Simone Doenges; Alexander C Langheinrich; Till Acker; Peter Reuter; Mesut Yeniguen; Jan Pukropski; Manfred Kaps; Clemens Mueller; Georg Bachmann; Tibo Gerriets |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-09 |
Journal Detail:
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Title: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Volume: 30 ISSN: 1559-7016 ISO Abbreviation: J. Cereb. Blood Flow Metab. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-04 Completed Date: 2010-10-27 Revised Date: 2011-10-03 |
Medline Journal Info:
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Nlm Unique ID: 8112566 Medline TA: J Cereb Blood Flow Metab Country: United States |
Other Details:
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Languages: eng Pagination: 1712-20 Citation Subset: IM |
Affiliation:
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Department of Neurology, Justus Liebig University, Giessen, Germany. max.nedelmann@neuro.med.uni-giessen.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Contrast Media / therapeutic use* Fibrinolytic Agents / therapeutic use* Infarction, Middle Cerebral Artery / drug therapy*, pathology Magnetic Resonance Imaging Male Microcirculation / drug effects* Rats Rats, Wistar Reperfusion Stroke / drug therapy*, pathology Thrombolytic Therapy / methods* Tissue Plasminogen Activator / therapeutic use* Ultrasonography / methods* |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media; 0/Fibrinolytic Agents; EC 3.4.21.68/Tissue Plasminogen Activator |
| Comments/Corrections | |
Erratum In:
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J Cereb Blood Flow Metab. 2011 Jul;31(7):1660 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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