Document Detail

Combined calcium fluorescence recording with ionic currents in contractile cells.
MedLine Citation:
PMID:  23007584     Owner:  NLM     Status:  In-Data-Review    
Measurement of calcium (Ca(2+)) fluorescence in conjunction with ionic currents is of particular importance in contractile cells, such as cardiac ventricular myocytes and vascular smooth muscle. The interplay between membrane potential and intracellular calcium ([Ca(2+)](i)) is fundamental to the regulation of contractile function and cell signalling. Here the loading of cells either with an esterified fluorescence indicator prior to patch clamp recording, or dye loading via the patch pipette with "free" indicator, is described to allow simultaneous measurement of fluorescence and electrical signals.
Richard D Rainbow
Related Documents :
8386224 - Contribution of g protein activation to fluoride stimulation of phosphoinositide hydrol...
25020554 - P134increased beta-adrenergic inotropy in ventricular myocardium from trpm4 knockout mice.
9207244 - Cooperation between the fc epsilonr1 and formyl peptide receptor signaling pathways in ...
24896114 - Frequency and relative prevalence of calcium blips and puffs in a model of small ip3r c...
6707924 - Comparative effects of calcium channel blocking agents and varying extracellular calciu...
6331434 - Nitrendipine and isoproterenol induce phosphorylation of a 42,000 dalton protein that c...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  937     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-09-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  149-60     Citation Subset:  IM    
Department of Cardiovascular Sciences, Leicester Royal Infirmary, University of Leicester, Leicester, UK,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Whole-cell patch-clamp recording of voltage-sensitive Ca²? channel currents in single cells: hetero...
Next Document:  Measurement of phospholipase C by monitoring inositol phosphates using [(3)h]inositol labeling proto...