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Combined calcium fluorescence recording with ionic currents in contractile cells.
MedLine Citation:
PMID:  23007584     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Measurement of calcium (Ca(2+)) fluorescence in conjunction with ionic currents is of particular importance in contractile cells, such as cardiac ventricular myocytes and vascular smooth muscle. The interplay between membrane potential and intracellular calcium ([Ca(2+)](i)) is fundamental to the regulation of contractile function and cell signalling. Here the loading of cells either with an esterified fluorescence indicator prior to patch clamp recording, or dye loading via the patch pipette with "free" indicator, is described to allow simultaneous measurement of fluorescence and electrical signals.
Authors:
Richard D Rainbow
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  937     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-09-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  149-60     Citation Subset:  IM    
Affiliation:
Department of Cardiovascular Sciences, Leicester Royal Infirmary, University of Leicester, Leicester, UK, rdr8@le.ac.uk.
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