| Combined autoradiography and immunohistochemistry for simultaneous localization of radioactively labeled steroid hormones and antibodies in the brain. | |
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MedLine Citation:
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PMID: 6895229 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A combined technique of autoradiography and immunohistochemistry is described for localization of radioactively labeled ligands and antibodies to neuropeptides, protein hormones and neurotransmitter synthesizing enzymes in the brain. This method permits the simultaneous visualization of radioactively labeled cells and neuropeptide-producing cells in the same tissue preparation. Autoradiograms are fixed with weak paraformaldehyde solution prior to photographic processing for subsequent immunoperoxidase staining. With this procedure 3H-estradiol is localized in neurophysin I and arginine vasopressin-producing cells of the mouse supraoptic and paraventricular nuclei, neurophysin-producing cells of the guinea pig hypothalamus, and dopamine-beta-hydroxylase-containing neurons of the rat lower brainstem; 3H-dihydrotestosterone in pituitary gonadotropes and thyrotropes; and 3H-1,25-(OH)2 vitamin D3 in pituitary thyrotropes and pancreatic B-cells. |
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Authors:
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M Sar; W E Stumpf |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 29 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 1981 Jan |
Date Detail:
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Created Date: 1981-12-21 Completed Date: 1981-12-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 161-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies* Autoradiography / methods* Brain / metabolism* Calcitriol / metabolism Dihydrotestosterone / metabolism Estradiol / metabolism Female Guinea Pigs Histocytochemistry Immunochemistry Pancreas / metabolism Pituitary Gland, Anterior / metabolism Rats Rats, Inbred Strains Steroids / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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NS09914/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Steroids; 32222-06-3/Calcitriol; 50-28-2/Estradiol; 521-18-6/Dihydrotestosterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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