Document Detail

Combined action of stem cell factor, leukemia inhibitory factor, and cAMP on in vitro proliferation of mouse primordial germ cells.
MedLine Citation:
PMID:  7686377     Owner:  NLM     Status:  MEDLINE    
In the present paper we investigated the effects of stem cell factor/mastocyte growth factor (SCF/MGF), leukemia inhibitory factor/differentiating inhibitory activity (LIF/DIA) (two growth factors known to affect primordial germ cell growth in vitro) and forskolin (FRSK) (an activator of adenylate cyclase in many cell types) alone or in combination on the survival and proliferation of primordial germ cells (PGCs) obtained from 8.5, 10.5, and 11.5 days post coitum (dpc) mouse embryos and cultured without pre-formed cell feeder layers. The results showed that both at 1 and 3 days of culture the addition of 100 ng/ml SCF, 20 microM FRSK, or in some instances 20 ng/ml LIF alone caused a significant increase of PGC number as compared with controls. The highest effects were obtained when SCF and/or LIF were used together with FRSK. Moreover, we found that FRSK elevated cAMP levels in purified 11.5 dpc PGCs and that this compound, but not SCF and LIF, stimulated PGC proliferation, as assessed by 5-bromo-2'-deoxyuridin (BrdU) incorporation. These results suggest a mechanism of combined action of cAMP with SCF and/or LIF in the control of proliferation of mouse PGCs in vitro.
S Dolci; M Pesce; M De Felici
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  35     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-08-05     Completed Date:  1993-08-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  134-9     Citation Subset:  IM    
Department of Public Health and Cell Biology, University of Rome Tor Vergata, Italy.
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MeSH Terms
Cell Division / drug effects
Cell Survival
Cells, Cultured
Cyclic AMP / analysis,  physiology*
Drug Synergism
Forskolin / pharmacology*
Germ Cells / drug effects*
Growth Inhibitors / pharmacology*
Hematopoietic Cell Growth Factors / pharmacology*
Leukemia Inhibitory Factor
Lymphokines / pharmacology*
Mice / embryology
Stem Cell Factor
Stem Cells / drug effects*
Reg. No./Substance:
0/Growth Inhibitors; 0/Hematopoietic Cell Growth Factors; 0/Interleukin-6; 0/Leukemia Inhibitory Factor; 0/Lif protein, mouse; 0/Lymphokines; 0/Stem Cell Factor; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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