| Combined SSCP and heteroduplex analysis of the human plasma membrane Ca(2+)-ATPase isoform 1 in patients with essential hypertension. | |
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MedLine Citation:
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PMID: 10425217 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In recent theories concerning the pathogenesis of essential hypertension, altered calcium homeostasis plays an important role. Increased intracellular Ca(2+) levels have repeatedly been reported in different cell types of hypertensive subjects. In vascular smooth muscle cells the plasma membrane Ca(2+)-ATPase (PMCA) represents the most important Ca(2+)-ejection system. Modifications of this pump therefore have been assumed to increase contractile tone of small vessels. For this reason, the purpose of this study was to determine if genetic alterations in the hPMCA1 gene might be associated with arterial hypertension. For detection of polymorphisms all 22 PMCA1 exons from 44 patients with essential hypertension (based on rigorous clinical data in addition to a positive family history) and from 40 normotensives without a family history of hypertension were PCR amplified and subsequently subjected to combined single-strand conformation polymorphism (SSCP) and heteroduplex (HTX) analysis. Despite the high sensitivity of almost 100%, differences could not be identified between hypertensives and normotensives within the two groups. These data indicate that at least in this population PMCA1 polymorphisms are presumably not related to common forms of essential hypertension. Furthermore, the high degree of evolutionary conservation of the PMCA1 gene underlines the pivotal role of this ATPase for cell physiology. |
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Authors:
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C Benkwitz; S Oberdorf-Maass; L Neyses |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 261 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1999 Aug |
Date Detail:
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Created Date: 1999-09-08 Completed Date: 1999-09-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 515-20 Citation Subset: IM |
Copyright Information:
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Copyright 1999 Academic Press. |
Affiliation:
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Department of Anesthesiology, University of Wuerzburg, Josef-Schneider-Strasse 2, Wuerzburg, D-97080, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Base Sequence Calcium / metabolism Calcium-Transporting ATPases / genetics* Case-Control Studies Cation Transport Proteins Cell Membrane / enzymology* DNA / genetics DNA Mutational Analysis DNA Primers / genetics Exons Female Humans Hypertension / enzymology*, genetics*, metabolism Isoenzymes / genetics* Male Middle Aged Nucleic Acid Heteroduplexes / genetics Plasma Membrane Calcium-Transporting ATPases Polymorphism, Single-Stranded Conformational* |
| Chemical | |
Reg. No./Substance:
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0/Cation Transport Proteins; 0/DNA Primers; 0/Isoenzymes; 0/Nucleic Acid Heteroduplexes; 7440-70-2/Calcium; 9007-49-2/DNA; EC 3.6.1.8/Calcium-Transporting ATPases; EC 3.6.3.8/ATP2B1 protein, human; EC 3.6.3.8/Plasma Membrane Calcium-Transporting ATPases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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