| Combined PI-DAPI staining (CPD) reveals NOR asymmetry and facilitates karyotyping of plant chromosomes. | |
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MedLine Citation:
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PMID: 10997779 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This paper presents a preparative and staining procedure for plant mitotic chromosomes that uses a combination of PI (propidium iodide) and DAPI (4',6-diamidino-2-phenylindol) and which reveals a pattern of high-affinity regions for these fluorochromes. Nucleolar organiser regions (NORs), telomeres and centromeric regions exhibit high PI affinity (red), whereas other chromosomal regions exhibit high affinity for either PI (red) or DAPI (blue). NOR-bearing and other chromosomes are readily distinguished, facilitating karyotyping. The dual staining pattern was observed in all the plants tested. Aspects of NOR size, number and occurrence are discussed. A karyotype of rice metaphase chromosomes is presented, based on their fluorescent banding patterns. |
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Authors:
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S C Andras; T P Hartman; J Alexander; R McBride; J A Marshall; J B Power; E C Cocking; M R Davey |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Volume: 8 ISSN: 0967-3849 ISO Abbreviation: Chromosome Res. Publication Date: 2000 |
Date Detail:
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Created Date: 2000-12-18 Completed Date: 2001-01-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9313452 Medline TA: Chromosome Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 387-91 Citation Subset: IM |
Affiliation:
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The Ecology Genetics Department, Faculty of Biology-Geology, University Babes-Bolyai, Cluj-Napoca, Romania. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Centromere
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ultrastructure Chromosome Banding Chromosomes Fluorescent Dyes / pharmacology* Genetic Techniques* Indicators and Reagents / pharmacology* Indoles / pharmacology* Karyotyping Mitosis Nucleolus Organizer Region / ultrastructure Oryza sativa / genetics Plants / genetics Propidium / pharmacology* Telomere / ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Indicators and Reagents; 0/Indoles; 36015-30-2/Propidium; 47165-04-8/DAPI |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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