Document Detail


Combined PI-DAPI staining (CPD) reveals NOR asymmetry and facilitates karyotyping of plant chromosomes.
MedLine Citation:
PMID:  10997779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper presents a preparative and staining procedure for plant mitotic chromosomes that uses a combination of PI (propidium iodide) and DAPI (4',6-diamidino-2-phenylindol) and which reveals a pattern of high-affinity regions for these fluorochromes. Nucleolar organiser regions (NORs), telomeres and centromeric regions exhibit high PI affinity (red), whereas other chromosomal regions exhibit high affinity for either PI (red) or DAPI (blue). NOR-bearing and other chromosomes are readily distinguished, facilitating karyotyping. The dual staining pattern was observed in all the plants tested. Aspects of NOR size, number and occurrence are discussed. A karyotype of rice metaphase chromosomes is presented, based on their fluorescent banding patterns.
Authors:
S C Andras; T P Hartman; J Alexander; R McBride; J A Marshall; J B Power; E C Cocking; M R Davey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  8     ISSN:  0967-3849     ISO Abbreviation:  Chromosome Res.     Publication Date:  2000  
Date Detail:
Created Date:  2000-12-18     Completed Date:  2001-01-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  387-91     Citation Subset:  IM    
Affiliation:
The Ecology Genetics Department, Faculty of Biology-Geology, University Babes-Bolyai, Cluj-Napoca, Romania.
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MeSH Terms
Descriptor/Qualifier:
Centromere / ultrastructure
Chromosome Banding
Chromosomes
Fluorescent Dyes / pharmacology*
Genetic Techniques*
Indicators and Reagents / pharmacology*
Indoles / pharmacology*
Karyotyping
Mitosis
Nucleolus Organizer Region / ultrastructure
Oryza sativa / genetics
Plants / genetics
Propidium / pharmacology*
Telomere / ultrastructure
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Indicators and Reagents; 0/Indoles; 36015-30-2/Propidium; 47165-04-8/DAPI

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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