Document Detail

Combined PI-DAPI staining (CPD) reveals NOR asymmetry and facilitates karyotyping of plant chromosomes.
MedLine Citation:
PMID:  10997779     Owner:  NLM     Status:  MEDLINE    
This paper presents a preparative and staining procedure for plant mitotic chromosomes that uses a combination of PI (propidium iodide) and DAPI (4',6-diamidino-2-phenylindol) and which reveals a pattern of high-affinity regions for these fluorochromes. Nucleolar organiser regions (NORs), telomeres and centromeric regions exhibit high PI affinity (red), whereas other chromosomal regions exhibit high affinity for either PI (red) or DAPI (blue). NOR-bearing and other chromosomes are readily distinguished, facilitating karyotyping. The dual staining pattern was observed in all the plants tested. Aspects of NOR size, number and occurrence are discussed. A karyotype of rice metaphase chromosomes is presented, based on their fluorescent banding patterns.
S C Andras; T P Hartman; J Alexander; R McBride; J A Marshall; J B Power; E C Cocking; M R Davey
Related Documents :
23106559 - Postmating isolation in six species of three genera (hoplobatrachus, euphlyctis and fej...
3943769 - Alpha-l-fucosidase phenotyping in human placentae, semen and seminal stains.
19443989 - Relations between cyclin d1 and chromosome 11 in thyroid carcinoma: analysis by dual st...
18521939 - Boundaries between subsegments iva and ivb in the human liver.
25186169 - Use of synteny to identify candidate genes underlying qtl controlling stomatal traits i...
2416669 - Simultaneous production of r-bands and either replication patterns or sister chromatid ...
22796359 - Genetics of early miscarriage.
23498939 - X-inactivation, imprinting, and long noncoding rnas in health and disease.
18413369 - Expansion in size of a terminal deletion: a paradigm shift for parental follow-up studies.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  8     ISSN:  0967-3849     ISO Abbreviation:  Chromosome Res.     Publication Date:  2000  
Date Detail:
Created Date:  2000-12-18     Completed Date:  2001-01-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  387-91     Citation Subset:  IM    
The Ecology Genetics Department, Faculty of Biology-Geology, University Babes-Bolyai, Cluj-Napoca, Romania.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Centromere / ultrastructure
Chromosome Banding
Fluorescent Dyes / pharmacology*
Genetic Techniques*
Indicators and Reagents / pharmacology*
Indoles / pharmacology*
Nucleolus Organizer Region / ultrastructure
Oryza sativa / genetics
Plants / genetics
Propidium / pharmacology*
Telomere / ultrastructure
Reg. No./Substance:
0/Fluorescent Dyes; 0/Indicators and Reagents; 0/Indoles; 36015-30-2/Propidium; 47165-04-8/DAPI

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Molecular organization of the Drosophila melanogaster Adh chromosomal region in D. repleta and D. bu...
Next Document:  Reciprocal chromosome painting illuminates the history of genome evolution of the domestic cat, dog ...