Document Detail


Combined effects of two environmental endocrine disruptors nonyl phenol and di-n-butyl phthalate on rat Sertoli cells in vitro.
MedLine Citation:
PMID:  20600820     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
In this study, our purpose is to analyze combined effects of nonyl phenol (NP) and di-n-butyl phthalate (DBP) for rat testicular Sertoli cell toxicity in vitro. Sertoli cells were isolated, purified, cultured, and identified with FSHR fluorescence staining. Then the purified Sertoli cells were treated with different doses of NP, DBP or NP+DBP. Although we did not find dramatic morphological changes, cell viability decreased significantly at high-dose NP and their mixture. The following Annexin V-PI staining demonstrated that DBP alone did not show apoptosis induction, the combination effect on apoptosis induction was due to NP, in addition, nucleus of Sertoli cell showed apoptosis morphological changes. In addition, increased LDH leakage was also observed in high-dose mixture. According to the above phenomena, we inferred that the combined effect of the two substances on Sertoli cell toxicity had an additive effect, and the induction of apoptosis may play an important role.
Authors:
Dongmei Li; Yang Hu; Xiahong Shen; Xinjue Dai; Xiaodong Han
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-25
Journal Detail:
Title:  Reproductive toxicology (Elmsford, N.Y.)     Volume:  30     ISSN:  1873-1708     ISO Abbreviation:  Reprod. Toxicol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-09-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8803591     Medline TA:  Reprod Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  438-45     Citation Subset:  IM    
Copyright Information:
(c) 2010. Published by Elsevier Inc.
Affiliation:
Immunology and Reproduction Biology Laboratory, Medical School, Nanjing University, Nanjing, Jiangsu 210093, China.
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