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The Combined Effects of Hematoporphyrin Monomethyl Ether-SDT and Doxorubicin on the Proliferation of QBC939 Cell Lines.
MedLine Citation:
PMID:  23200181     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
It is well established that both hematoporphyrin monomethyl ether-sonodynamic therapy (HMME-SDT) and doxorubicin (DOX) can induce cell apoptosis but each alone has its own limitations. To date, the combined effects of HMME-SDT and DOX on inducing cell apoptosis are little known and the mechanism for the combined effects remains poorly understood. In the present study, we reported the synergistic effects of HMME-SDT and DOX on inhibiting the proliferation of human cholangiocarcinoma QBC939 cells and investigated the mechanism of this synergy. The data from MTT assay, flow cytometer, Hoechst staining and cell arrest analysis showed that the combination of HMME-SDT and DOX exhibited higher inhibiting effects on proliferation of QBC939 cells than the sole application of HMME-SDT or DOX. In addition, the synergistic effects were shown to result from the DNA damage as demonstrated by single cell gel electrophoresis and DNA fragmentation. Furthermore, the expression of p53, Fas, Bax and activated caspase-3 protein was significantly upregulated in cells treated with HMME-SDT and DOX, whereas Bcl-2 protein was downregulated. Taken together, our data suggested that the application of HMME-SDT combined with DOX had better inhibiting effects on QBC939 cells and the effects were caused mainly by DNA damage.
Authors:
Lei Liang; Sheng Xie; Lin Jiang; Hui Jin; Songgang Li; Jianwen Liu
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Ultrasound in medicine & biology     Volume:  39     ISSN:  1879-291X     ISO Abbreviation:  Ultrasound Med Biol     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0410553     Medline TA:  Ultrasound Med Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  146-60     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.
Affiliation:
State Key Laboratory of Bioreactor Engineering and Shanghai Key Laboratory of Chemical Biology, School of pharmacy, East China University of Science and Technology, Shanghai, People's Republic of China.
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