Document Detail

Combined effect of AMPK/PPAR agonists and exercise training in mdx mice functional performance.
MedLine Citation:
PMID:  23029189     Owner:  NLM     Status:  MEDLINE    
The present investigation was undertaken to test whether exercise training (ET) associated with AMPK/PPAR agonists (EM) would improve skeletal muscle function in mdx mice. These drugs have the potential to improve oxidative metabolism. This is of particular interest because oxidative muscle fibers are less affected in the course of the disease than glycolitic counterparts. Therefore, a cohort of 34 male congenic C57Bl/10J mdx mice included in this study was randomly assigned into four groups: vehicle solution (V), EM [AICAR (AMPK agonist, 50 mg/, ip) and GW 1516 (PPARδ agonist, 2.5 mg/, gavage)], ET (voluntary running on activity wheel) and EM+ET. Functional performance (grip meter and rotarod), aerobic capacity (running test), muscle histopathology, serum creatine kinase (CK), levels of ubiquitined proteins, oxidative metabolism protein expression (AMPK, PPAR, myoglobin and SCD) and intracellular calcium handling (DHPR, SERCA and NCX) protein expression were analyzed. Treatments started when the animals were two months old and were maintained for one month. A significant functional improvement (p<0.05) was observed in animals submitted to the combination of ET and EM. CK levels were decreased and the expression of proteins related to oxidative metabolism was increased in this group. There were no differences among the groups in the intracellular calcium handling protein expression. To our knowledge, this is the first study that tested the association of ET with EM in an experimental model of muscular dystrophy. Our results suggest that the association of ET and EM should be further tested as a potential therapeutic approach in muscular dystrophies.
Carlos R Bueno Júnior; Lucas C Pantaleão; Vanessa A Voltarelli; Luiz Henrique M Bozi; Patricia Chakur Brum; Mayana Zatz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-21
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-02     Completed Date:  2013-03-28     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e45699     Citation Subset:  IM    
Human Genome Research Center - Institute of Biosciences, University of Sao Paulo, Sao Paulo, Brazil.
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MeSH Terms
Adenylate Kinase / drug effects*
Adipose Tissue
Calcium / metabolism
Kidney Function Tests
Mice, Inbred C57BL
Mice, Inbred mdx
Muscle Proteins / metabolism
Muscle, Skeletal / metabolism,  pathology
Peroxisome Proliferator-Activated Receptors / agonists*
Physical Conditioning, Animal*
Reg. No./Substance:
0/Muscle Proteins; 0/Peroxisome Proliferator-Activated Receptors; 7440-70-2/Calcium; EC Kinase

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