| Combinatorial, selective and reversible control of gene expression using oligodeoxynucleotides in a cell-free protein synthesis system. | |
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MedLine Citation:
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PMID: 18767195 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Herein we describe the methods for selective and reversible regulation of gene expression using antisense oligodeoxynucleotides (ODNs) in a cell-free protein synthesis system programmed with multiple DNAs. Either a complete shut down or controlled level of gene expression was attained through the antisense ODN-mediated regulation of mRNA stability in the reaction mixture. In addition to the primary control of gene expression, we also demonstrate that the inhibition of protein synthesis can be reversed by using an anti-antisense ODN sequence that strips the antisense ODN off the target sequence of mRNA. As a result, sequential additions of the antisense and anti-antisense ODNs enabled the stop-and-go expression of protein molecules. Through the on-demand regulation of gene expression, presented results will provide a versatile platform for the analysis and understanding of the complicated networks of biological components. |
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Authors:
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Jung-Won Keum; Jin-Ho Ahn; Taek Jin Kang; Dong-Myung Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 102 ISSN: 1097-0290 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2008-12-29 Completed Date: 2009-01-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 577-82 Citation Subset: IM |
Affiliation:
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School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Seoul, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biotechnology
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methods* Cell Culture Techniques Cell-Free System Cloning, Molecular Gene Expression Regulation* Green Fluorescent Proteins / genetics Oligodeoxyribonucleotides, Antisense / genetics* Protein Biosynthesis / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Oligodeoxyribonucleotides, Antisense; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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