Document Detail

Combinatorial code of growth factors and neuropeptides define neuroendocrine differentiation in PC12 cells.
MedLine Citation:
PMID:  14637105     Owner:  NLM     Status:  MEDLINE    
Adrenal chromaffin cells constitute one of the first cell types to have been defined as a neuroendocrine cell type. Since they produce dopamine, these cells have been proposed for the treatment of neuronal deficits in human Parkinson's disease. However, the factors involved in the development of chromaffin cells are still poorly understood. Based on recent insights from stem cell research, we decided to study the role of extracellular matrices, growth factors and neuropeptides on the neuroendocrine differentiation in a serum-free medium of PC12 cells. Employing immunohistochemistry, quantitative PCR and HPLC analysis, neuroendocrine differentiation was determined by evaluating neurite outgrowth, catecholamine biosynthesis and release as well as neuropeptide and vesicular protein mRNA expression. The combination of bFGF, NGF and PACAP could prevent the inhibition of neurite process development induced by dexamethasone in PC12 cells cultured on ECM. Whereas glucocorticoids were essential in the regulation of enzymes of catecholamine biosynthesis and metabolism, growth factors and PACAP were more efficient in inducing neuropeptide and chromogranin B expression as well as release of dopamine and 3-methoxytyramine. Therefore, in addition to glucocorticoids, chromaffin cells need a gradient of matrix, growth factors, and neuropeptides to develop the full functional phenotype of a neuroendocrine cell.
Delphine Beaujean; Claudia Rosenbaum; Hans-Werner Müller; Jacques J Willemsen; Jacques Lenders; Stefan R Bornstein
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental neurology     Volume:  184     ISSN:  0014-4886     ISO Abbreviation:  Exp. Neurol.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-11-25     Completed Date:  2004-01-05     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0370712     Medline TA:  Exp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  348-58     Citation Subset:  IM    
Department of Endocrinology, University of Düsseldorf, 40225, Düsseldorf, Germany.
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MeSH Terms
Catecholamines / biosynthesis,  metabolism
Cell Differentiation / physiology
Cell Survival / physiology
Chromatography, High Pressure Liquid
Culture Media, Serum-Free
Dexamethasone / pharmacology
Extracellular Matrix / physiology
Growth Substances / biosynthesis*
Nerve Tissue Proteins / biosynthesis
Neurites / physiology
Neuropeptides / biosynthesis*,  pharmacology
Neurosecretory Systems / cytology,  physiology*
PC12 Cells
Pituitary Adenylate Cyclase-Activating Polypeptide
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Adcyap1 protein, rat; 0/Catecholamines; 0/Culture Media, Serum-Free; 0/Growth Substances; 0/Nerve Tissue Proteins; 0/Neuropeptides; 0/Pituitary Adenylate Cyclase-Activating Polypeptide; 0/RNA, Messenger; 50-02-2/Dexamethasone

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