Document Detail

Combinatorial biochemistry in plants: the case of O-methyltransferases.
MedLine Citation:
PMID:  11198815     Owner:  NLM     Status:  MEDLINE    
Combinatorial chemistry is common place today in chemical synthesis. Virtually thousands of derivatives of a molecule can be achieved by automated systems. The use of biological systems to exploit combinatorial chemistry (combinatorial biochemistry) now has multiple examples in the polyketide field. The modular functional domain structure of polyketide synthases have been recombined through genetic engineering into unnatural constellations in heterologous hosts in order to produce polyketide structures not yet discovered in nature. We present herein an example for a potential type of combinatorial biochemistry in alkaloidal systems using various combinations of Thalictrum tuberosum (meadow rue) O-methyltransferase subunits that result in heterodimeric enzymes with substrate specificities that differ from those of the homodimeric native enzymes.
S Frick; A Ounaroon; T M Kutchan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytochemistry     Volume:  56     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-01-25     Completed Date:  2001-04-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-4     Citation Subset:  IM    
Leibniz-lnstitut für Pflanzenbiochemie, Halle/Saale, Germany.
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MeSH Terms
Angiosperms / enzymology,  metabolism*
Combinatorial Chemistry Techniques*
Methyltransferases / chemistry,  metabolism*
Substrate Specificity
Reg. No./Substance:
EC 2.1.1.-/Methyltransferases

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