| Combinatorial Domain Hunting: solving problems in protein expression. | |
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MedLine Citation:
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PMID: 20338258 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Modern drug discovery demands large amounts of high-quality protein and, therefore, begins by expressing target genes in heterologous systems such as bacteria or insect cells. However, some of the most attractive drug targets have proven challenging to clone and express. A technology called Combinatorial Domain Hunting has been developed to express regions or domains of proteins in a non-aggregated form. This technology has been applied to more than 50 targets and, in all cases, high-expressing protein domains have been produced. In many cases, the protein is also non-aggregated, making it ideal for developing assays for screening or determining its X-ray crystal structure. |
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Authors:
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Eddy Littler |
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Publication Detail:
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Type: Journal Article; Review Date: 2010-03-23 |
Journal Detail:
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Title: Drug discovery today Volume: 15 ISSN: 1878-5832 ISO Abbreviation: Drug Discov. Today Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-08-05 Completed Date: 2010-11-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9604391 Medline TA: Drug Discov Today Country: England |
Other Details:
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Languages: eng Pagination: 461-7 Citation Subset: IM |
Copyright Information:
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(c) 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Domainex, 324 Cambridge Science Park, Milton Road, Cambridge, CB4 0WG, UK. eddy.littler@domainex.co.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Combinatorial Chemistry Techniques* Crystallography, X-Ray Drug Delivery Systems Drug Discovery / methods* Gene Expression Humans Protein Conformation Proteins / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Proteins |
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