Document Detail


Combination of nanogel polyethylene glycol-polyethylenimine and 6(hydroxymethyl)-1,4-anthracenedione as an anticancer nanomedicine.
MedLine Citation:
PMID:  18572646     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polyethylene glycol-polyethylenimine (PEG-PEI) nanogels have been used to deliver nucleic acids and oligonucleotides into cells. First, we synthesized PEG-PEI nanogels with methylene proton ratios (CH2O:CH2N) in PEG-PEI ranging from approximately 6.8:1 to 4:1 and less, as shown by 1H NMR spectra. We first synthesized various nanogels with varying ratios of CH2O:CH2N (methylene proton) in PEG-PEI as shown by 1H NMR spectra and tested their cytotoxicity using a rodent pancreatic adenocarcinoma cell line (Pan 02). We showed that the nanogel PEG-PEI with methylene proton ratio of 4:1 was strongly cytotoxic to Pan 02 cells in vitro, while the nanogel with the methylene proton ratio of 6.8:1 was not toxic. We incorporated a novel anti-cancer drug, 6-(hydroxymethyl)-1,4-anthracenedione (AQ) analogue (AQ10) into nontoxic nanogel PEG-PEI and tested the effect of AQ10 loaded nanogel PEG-PEI (AQ10-nanogel PEG-PEI) and AQ10 dissolved in DMSO on Pan 02 cell growth. The size of this AQ10-nanogel PEG-PEI was characterized using atomic force microscopy (AFM). Our studies showed that the AQ10-nanogel PEG-PEI is readily taken up by Pan 02 cells. Growth attenuation of Pan 02 cells treated with AQ10-nanogel PEG-PEI was three to four times that of cells treated with AQ10 dissolved in DMSO. These results suggest that PEG-PEI, usually used to deliver nucleic acids into cells, can also be used to deliver an insoluble small molecule anticancer drug, AQ10.
Authors:
Chanran Ganta; Aibin Shi; Srinivas K Battina; Marla Pyle; Sandeep Rana; Duy H Hua; Masaaki Tamura; Deryl Troyer
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of nanoscience and nanotechnology     Volume:  8     ISSN:  1533-4880     ISO Abbreviation:  J Nanosci Nanotechnol     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-06-24     Completed Date:  2008-08-13     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  101088195     Medline TA:  J Nanosci Nanotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2334-40     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anthraquinones / administration & dosage*
Antineoplastic Agents / administration & dosage*
Cell Proliferation
Gels
Nanomedicine*
Neoplasms / therapy*
Polyethylene Glycols / administration & dosage*
Polyethyleneimine / administration & dosage*
Rodentia
Grant Support
ID/Acronym/Agency:
R01 AG025500-01A2/AG/NIA NIH HHS; R01 AG025500-02/AG/NIA NIH HHS; R01AG025500/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/6(hydroxymethyl)-1,4-anthracenedione; 0/Anthraquinones; 0/Antineoplastic Agents; 0/Gels; 0/Polyethylene Glycols; 9002-98-6/Polyethyleneimine
Comments/Corrections

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