| Combination of nanogel polyethylene glycol-polyethylenimine and 6(hydroxymethyl)-1,4-anthracenedione as an anticancer nanomedicine. | |
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MedLine Citation:
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PMID: 18572646 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Polyethylene glycol-polyethylenimine (PEG-PEI) nanogels have been used to deliver nucleic acids and oligonucleotides into cells. First, we synthesized PEG-PEI nanogels with methylene proton ratios (CH2O:CH2N) in PEG-PEI ranging from approximately 6.8:1 to 4:1 and less, as shown by 1H NMR spectra. We first synthesized various nanogels with varying ratios of CH2O:CH2N (methylene proton) in PEG-PEI as shown by 1H NMR spectra and tested their cytotoxicity using a rodent pancreatic adenocarcinoma cell line (Pan 02). We showed that the nanogel PEG-PEI with methylene proton ratio of 4:1 was strongly cytotoxic to Pan 02 cells in vitro, while the nanogel with the methylene proton ratio of 6.8:1 was not toxic. We incorporated a novel anti-cancer drug, 6-(hydroxymethyl)-1,4-anthracenedione (AQ) analogue (AQ10) into nontoxic nanogel PEG-PEI and tested the effect of AQ10 loaded nanogel PEG-PEI (AQ10-nanogel PEG-PEI) and AQ10 dissolved in DMSO on Pan 02 cell growth. The size of this AQ10-nanogel PEG-PEI was characterized using atomic force microscopy (AFM). Our studies showed that the AQ10-nanogel PEG-PEI is readily taken up by Pan 02 cells. Growth attenuation of Pan 02 cells treated with AQ10-nanogel PEG-PEI was three to four times that of cells treated with AQ10 dissolved in DMSO. These results suggest that PEG-PEI, usually used to deliver nucleic acids into cells, can also be used to deliver an insoluble small molecule anticancer drug, AQ10. |
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Authors:
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Chanran Ganta; Aibin Shi; Srinivas K Battina; Marla Pyle; Sandeep Rana; Duy H Hua; Masaaki Tamura; Deryl Troyer |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of nanoscience and nanotechnology Volume: 8 ISSN: 1533-4880 ISO Abbreviation: J Nanosci Nanotechnol Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-06-24 Completed Date: 2008-08-13 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 101088195 Medline TA: J Nanosci Nanotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 2334-40 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anthraquinones / administration & dosage* Antineoplastic Agents / administration & dosage* Cell Proliferation Gels Nanomedicine* Neoplasms / therapy* Polyethylene Glycols / administration & dosage* Polyethyleneimine / administration & dosage* Rodentia |
| Grant Support | |
ID/Acronym/Agency:
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R01 AG025500-01A2/AG/NIA NIH HHS; R01 AG025500-02/AG/NIA NIH HHS; R01AG025500/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/6(hydroxymethyl)-1,4-anthracenedione; 0/Anthraquinones; 0/Antineoplastic Agents; 0/Gels; 0/Polyethylene Glycols; 9002-98-6/Polyethyleneimine |
| Comments/Corrections | |
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