| Combination effects of normobaric hyperoxia and edaravone on focal cerebral ischemia-induced neuronal damage in mice. | |
| | |
MedLine Citation:
|
PMID: 18577423 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We evaluated the potential neuroprotective effects of combination treatment with normobaric hyperoxia (NBO) and edaravone, a potent scavenger of hydroxyl radicals, on acute brain injuries after stroke. Mice subjected to 2-h filamental middle cerebral artery occlusion were treated with NBO (95% O2, during the ischemia) alone, with edaravone (1.5 mg/kg, intravenously after the ischemia) alone, with both of these treatments (combination), or with vehicle. The histological and neurological score were assessed at 22-h after reperfusion. Infarct volume was significantly reduced in the combination group [36.3+/-6.7 mm3 (n=10) vs. vehicle: 65.5+/-5.9 mm3 (n=14) P<0.05], but not in the two monotherapy-groups [NBO: 50.5+/-5.8 mm3 (n=14) and edaravone: 56.7+/-5.8 mm3 (n=10)]. The combination therapy reduced TUNEL-positive cells in the ischemic boundary zone both in cortex [6.0+/-1.4 x 10(2)/mm2 (n=5) vs. vehicle: 18.9+/-2.4 x 10(2)/mm2 (n=5), P<0.01] and subcortex [11.6+/-1.5 x 10(2)/mm2 (n=5) vs. vehicle: 22.5+/-2.1 x 10(2)/mm2 (n=5), P<0.01]. NBO and combination groups exhibited significantly reduced neurological deficit scores at 22-h after reperfusion (vs. vehicle, P<0.05). Combination therapy with NBO plus edaravone prevented the neuronal damage after focal cerebral ischemia and reperfusion in mice, compared with monotherapy of NBO or edaravone. |
| | |
Authors:
|
Yuko Nonaka; Masamitsu Shimazawa; Shinichi Yoshimura; Toru Iwama; Hideaki Hara |
Related Documents
:
|
2589193 - Clinical correlates and prognostic significance of type a behavior and silent myocardia... 16778133 - Dynamic responses of single cardiomyocytes to graded ischemia studied by oxygen clamp i... 8078883 - Cytoplasmic transfer of the mtdna nt 8993 t-->g (atp6) point mutation associated with l... |
Publication Detail:
|
Type: Journal Article Date: 2008-06-18 |
Journal Detail:
|
Title: Neuroscience letters Volume: 441 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 2008 Aug |
Date Detail:
|
Created Date: 2008-07-15 Completed Date: 2008-11-05 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: Ireland |
Other Details:
|
Languages: eng Pagination: 224-8 Citation Subset: IM |
Affiliation:
|
Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antipyrine / analogs & derivatives*, therapeutic use Blood Pressure / drug effects Cell Death / drug effects Cerebral Infarction / etiology, pathology*, therapy* Disease Models, Animal Free Radical Scavengers / therapeutic use* Hyperbaric Oxygenation / methods* In Situ Nick-End Labeling / methods Infarction, Middle Cerebral Artery / complications Male Mice Neurologic Examination Neurons / drug effects, pathology* Tetrazolium Salts / diagnostic use |
| Chemical | |
Reg. No./Substance:
|
0/Free Radical Scavengers; 0/Tetrazolium Salts; 60-80-0/Antipyrine; 89-25-8/phenylmethylpyrazolone; 902-00-1/triphenyltetrazolium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The spectral power coherence of the EEG under different EMF conditions.
Next Document: Expression of the neuron-specific potassium chloride cotransporter KCC2 in adult rat cochlear.