| Combination of amino acids in the 3a protein and the coat protein of cucumber mosaic virus determines symptom expression and viral spread in bottle gourd. | |
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MedLine Citation:
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PMID: 11402857 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bottle gourd plants infected with an isolate of cucumber mosaic virus (CMV-KM) developed severe chronic mosaic symptoms (SCMS) with stunting, but two other isolates (CMV-Y and CMV-D8) did not. CMV-KM and CMV-D8 induced enlarged chlorotic spots and rapidly spread over the inoculated cotyledons, whereas CMV-Y elicited a hypersensitive response (HR) producing pin-point necrotic lesions. Reassortment analysis among the three isolates revealed that the local and systemic symptoms on the plants were regulated by RNA3. Reciprocal recombination and site-directed point mutation analyses of the three RNA3s demonstrated that a combination of genetic information encoded by the movement protein (MP) gene and the coat protein (CP) gene determines the induction of SCMS in bottle gourd. SCMS occurred when Ser51 in the MP of CMV-D8 was changed to Asn51, whereas substitution of Ser51 for Asn51 in the MP of CMV-KM eliminated its ability to induce SCMS. Furthermore, Ser129 in the CPs was shown to be responsible for induction of HR and blocking of efficient cell-to-cell and long-distance movement. |
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Authors:
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M Takeshita; M Suzuki; Y Takanami |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of virology Volume: 146 ISSN: 0304-8608 ISO Abbreviation: Arch. Virol. Publication Date: 2001 |
Date Detail:
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Created Date: 2001-06-13 Completed Date: 2001-07-05 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7506870 Medline TA: Arch Virol Country: Austria |
Other Details:
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Languages: eng Pagination: 697-711 Citation Subset: IM |
Affiliation:
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Laboratory of Plant Pathology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Capsid
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genetics*,
physiology* Capsid Proteins* Cucumovirus / genetics, pathogenicity* Cucurbitaceae / virology* Genes, Viral Kinetics Mutagenesis, Site-Directed Plant Diseases / virology Plant Leaves / virology Plant Viral Movement Proteins Point Mutation RNA, Viral / biosynthesis, genetics Reassortant Viruses / genetics, pathogenicity Species Specificity Viral Proteins / genetics*, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Capsid Proteins; 0/Plant Viral Movement Proteins; 0/RNA, Viral; 0/Viral Proteins; 156289-31-5/RNA 3 coat protein, prune dwarf ilarvirus |
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