| Combination of GLP-1 and sodium butyrate promote differentiation of pancreatic progenitor cells into insulin-producing cells. | |
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MedLine Citation:
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PMID: 18573514 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We identified that nestin-EGFP-positive cells isolated by fluorescent-activated cell sorting (FACS) were downstream progenitor cells from colony-initiating pancreatic precursor cells. Under differentiation condition, these nestin-EGFP-positive cells could generate islet and neural cells. To investigate the conditions that allowed nestin-EGFP-positive progenitor cells (NPPCs) to efficaciously differentiate into insulin-producing cells in vitro, protocols were designed with glucagon-like peptide-1 (GLP-1) and the histone deacetylase inhibitor, sodium butyrate. We demonstrated that the combination of GLP-1 and sodium butyrate resulted in the increasing of levels of transcripts encoding pancreatic developmental factors and insulin. As a consequence, the amount of insulin-producing cells and insulin secretion were enhanced. These results indicated that NPPCs which were cultured in the presence of GLP-1 and sodium butyrate could specially differentiate into insulin-producing cells. |
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Authors:
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Li Li; Ren Lili; Qi Hui; Wang Min; Wen Xue; Shen Xin; Liu Jing; Li Yan; Liu Yeqiang; He Fenrong; Li Furong; Shen Guanxin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-06-24 |
Journal Detail:
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Title: Tissue & cell Volume: 40 ISSN: 0040-8166 ISO Abbreviation: Tissue Cell Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-10-28 Completed Date: 2009-01-09 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0214745 Medline TA: Tissue Cell Country: Scotland |
Other Details:
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Languages: eng Pagination: 437-45 Citation Subset: IM |
Affiliation:
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Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / metabolism Butyrates / pharmacology* Cell Culture Techniques / methods Cell Differentiation / drug effects Female Glucagon-Like Peptide 1 / pharmacology* Green Fluorescent Proteins / genetics Histone Deacetylase Inhibitors* Insulin / metabolism Insulin-Secreting Cells / cytology*, physiology Intermediate Filament Proteins / genetics Male Mice Mice, Inbred C57BL Mice, Transgenic Nerve Tissue Proteins / genetics Receptors, Glucagon / metabolism Stem Cells / cytology*, drug effects, physiology |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Butyrates; 0/Histone Deacetylase Inhibitors; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/Receptors, Glucagon; 0/glucagon-like peptide receptor; 0/nestin; 11061-68-0/Insulin; 147336-22-9/Green Fluorescent Proteins; 89750-14-1/Glucagon-Like Peptide 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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