| Combination of fluorescence in situ hybridization with staining techniques for cell viability and accumulation of PHA and polyP in microorganisms in complex microbial systems. | |
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MedLine Citation:
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PMID: 19882282 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorescence in situ hybridization (FISH) can be combined with a number of staining techniques to reveal the relationships between the microorganisms and their function in complex microbial systems with a single-cell resolution. In this chapter, we have focused on staining methods for intracellular storage compounds (polyhydroxyalkanoates, polyphosphate) and a measure for cell viability, reduction of the tetrazolium-based redox stain CTC. These protocols are optimized for the study of microorganisms in waste-water treatment (activated sludge and biofilms), but they may also be used with minor modifications in many other ecosystems. |
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Authors:
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Jeppe Lund Nielsen; Caroline Kragelund; Per Halkjaer Nielsen |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 599 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2009-11-02 Completed Date: 2010-02-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 103-16 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biodegradation, Environmental* Environmental Microbiology* In Situ Hybridization, Fluorescence / methods* Polyhydroxyalkanoates / metabolism* Polyphosphates / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Polyhydroxyalkanoates; 0/Polyphosphates |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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