Document Detail


Combination of fluorescence in situ hybridization with staining techniques for cell viability and accumulation of PHA and polyP in microorganisms in complex microbial systems.
MedLine Citation:
PMID:  19882282     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fluorescence in situ hybridization (FISH) can be combined with a number of staining techniques to reveal the relationships between the microorganisms and their function in complex microbial systems with a single-cell resolution. In this chapter, we have focused on staining methods for intracellular storage compounds (polyhydroxyalkanoates, polyphosphate) and a measure for cell viability, reduction of the tetrazolium-based redox stain CTC. These protocols are optimized for the study of microorganisms in waste-water treatment (activated sludge and biofilms), but they may also be used with minor modifications in many other ecosystems.
Authors:
Jeppe Lund Nielsen; Caroline Kragelund; Per Halkjaer Nielsen
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  599     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2009-11-02     Completed Date:  2010-02-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  103-16     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biodegradation, Environmental*
Environmental Microbiology*
In Situ Hybridization, Fluorescence / methods*
Polyhydroxyalkanoates / metabolism*
Polyphosphates / metabolism*
Chemical
Reg. No./Substance:
0/Polyhydroxyalkanoates; 0/Polyphosphates

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis to Assess Microbial Commun...
Next Document:  Ecophysiological Analysis of Microorganisms in Complex Microbial Systems by Combination of Fluoresce...