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A Combination of CD15/CD10, CD64/CD33, CD16/CD13 or CD11b Flow Cytometric Granulocyte Panels is Sensitive and Specific for Diagnosis of Myelodysplastic Syndrome.
MedLine Citation:
PMID:  22964615     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Flow cytometry (FCM) is a reproducible and objective technique that may be useful in the diagnosisof myelodysplastic syndrome (MDS) by detecting abnormal immunophenotypes specific to MDS. We investigated 5 granulocyte/monocyte panels by FCM to find a sensitive and specific combination of panels in order to discriminate MDS from non-clonal hematologic disorders. Bone marrow aspirates from 35 patients with MDS and 25 patients with non-clonal hematologic disorders were studied. We performed FCM using 5 granulocyte/monocyte panels (CD15/CD10/CD45, CD64/CD33/CD45, CD16/CD13/CD45, CD16/CD11b/CD45, and CD56/CD19/CD7/CD45) to examine the positive rate in MDS and controls, and to find an optimal combination that maximizes the detection rate of MDS. In MDS, the number of abnormal immunophenotypes per 5 granulocytic and 5 monocytic panels were 2.1±1.2 and 2.2±1.4. The rates were higher than the controls (P< 0.001, respectively). As the number of employed panels increased, the percent values of abnormal immunophenotypes increased (P=0.002). The maximum rate of abnormal immunophenotype was 89.7% in MDS patients, especially 100.0% in normal karyotype, when a combination of three panels, CD15/CD10/CD45, CD64/CD33/CD45, and either CD16/CD13/CD45 or CD16/CD11b/CD45 was used. This study demonstrates that a combination of CD15/CD10, CD64/CD33, CD16/CD13 or CD11b granulocyte panels in FCM is sensitive and specific for diagnosis of MDS.
Authors:
Jae-Woo Chung; Chan-Jeoung Park; Choong-Hwan Cha; Young-Uk Cho; Seongsoo Jang; Hyun-Sook Chi; Eul-Ju Seo; Jung-Hee Lee; Je-Hwan Lee; Kyoo-Hyung Lee; Ho Joon Im; Jong-Jin Seo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of clinical and laboratory science     Volume:  42     ISSN:  1550-8080     ISO Abbreviation:  Ann. Clin. Lab. Sci.     Publication Date:  2012  
Date Detail:
Created Date:  2012-09-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0410247     Medline TA:  Ann Clin Lab Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  271-80     Citation Subset:  IM    
Affiliation:
Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center; 88, Olympic-Ro 43-Gil, Songpa- Gu, Seoul 138-736 (Republic of Korea). cjpark@amc.seoul.kr.
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