Document Detail


A ComGA-dependent checkpoint limits growth during the escape from competence.
MedLine Citation:
PMID:  11298275     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In Bacillus subtilis, competence for transformation develops in 5-10% of the cells in a stationary phase culture. These cells exhibit a prolonged lag in the resumption of growth and cell division during the escape from competence. To better understand the basis of this lag, we have characterized competent cultures microscopically. To distinguish the minority of competent cells, a translational fusion between ComK, the competence transcription factor, and the green fluorescent protein (GFP) was used as a marker. Only 5-10% of the cells in a competent culture were fluorescent, indicating that ComK synthesis is an all or nothing event. To validate the identification of competent cells, we demonstrated the coincident expression of comEA, a late competence gene, and comK-gfp. Competent cells resemble stationary phase cells; the majority are single (not in chains), contain single nucleoids, and rarely contain FtsZ rings. Upon dilution into fresh medium, competent cells maintain this appearance for about 2 h. In contrast, the majority of non-competent cells rapidly resume growth, exhibiting chaining, nuclear division and FtsZ-ring formation. The late competence protein ComGA is required for the competence-related block in chromosome replication and cell division. In the competent cells of a comGA mutant culture, chromosomal replication and FtsZ-ring formation were no longer blocked, although competent comGA mutant cells were abnormal in appearance. It is likely that one role for ComGA is to prevent growth, chromosome replication and cell division until ComK can be eliminated by degradation. A mutation in the ATP-binding site of comGA inactivated the protein for transformation but did not prevent it from inhibiting DNA replication and cell division. The buoyant density difference between competent and non-competent cells depends on the competence-specific growth arrest.
Authors:
B J Haijema; J Hahn; J Haynes; D Dubnau
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular microbiology     Volume:  40     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-04-12     Completed Date:  2001-06-14     Revised Date:  2011-08-12    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  52-64     Citation Subset:  IM    
Affiliation:
Public Health Research Institute, 455 First Ave, New York, NY 10016, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacillus subtilis / cytology,  growth & development*
Bacterial Proteins / genetics,  metabolism*
Base Sequence
Cell Division
DNA Primers
Green Fluorescent Proteins
Luminescent Proteins / metabolism
Mutation
Phenotype
Recombinant Fusion Proteins / metabolism
Grant Support
ID/Acronym/Agency:
GM 5720/GM/NIGMS NIH HHS; GM43756/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA Primers; 0/Luminescent Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections
Comment In:
Mol Microbiol. 2011 Jul;81(1):1-3   [PMID:  21595759 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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