Document Detail

Colorimetric viral detection based on sialic acid stabilized gold nanoparticles.
MedLine Citation:
PMID:  23208092     Owner:  NLM     Status:  MEDLINE    
Sialic acid reduced and stabilized gold nanoparticles (d=20.1±1.8 nm) were synthesized by a simple one-pot, green method without chemically modifying sialic acid for colorimetric detection of influenza virus. The gold nanoparticles showed target-specific aggregation with viral particles via hemagglutinin-sialic acid binding. A linear correlation was observed between the change in optical density and dilution of chemically inactivated influenza B/Victoria and influenza B/Yamagata. Virus dilution (hemagglutination assay titer, 512) of 0.156 vol% was readily detected. The upper limit of the linearity can be extended with the use of more sialic acid-gold nanoparticles.
Changwon Lee; Marsha A Gaston; Alison A Weiss; Peng Zhang
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-11-06
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  42     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-02-15     Completed Date:  2013-09-03     Revised Date:  2014-04-15    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  236-41     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
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MeSH Terms
Gold / chemistry*
Hemagglutinins / chemistry
Metal Nanoparticles / chemistry*
N-Acetylneuraminic Acid / chemical synthesis,  chemistry
Orthomyxoviridae / isolation & purification*
Virion / isolation & purification*
Grant Support
Reg. No./Substance:
0/Hemagglutinins; 7440-57-5/Gold; GZP2782OP0/N-Acetylneuraminic Acid

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