Document Detail

Colorimetric and fluorometric substrate immunoassays for detection of Salmonella in all foods: comparative study.
MedLine Citation:
PMID:  2289929     Owner:  NLM     Status:  MEDLINE    
Two modified fluorescent enzyme immunoassays for the detection of Salmonella in food have been developed. Both of the new procedures, which substitute a colorimetric substrate for the fluorescent substrate and in which results are read visually or with a photometer, are modifications of AOAC method 989.15. The visually read procedure uses the same antibody-coated wells as in method 989.15. The colorimetric end point of the assay is determined by comparing the solution color to a color chart. The assay result may also be read in a photometer, if the solution is first transferred to a transparent microtiter well. The second procedure designed to be read in a photometer substitutes clear, antibody-coated wells for those used in the fluorescent assay. The colorimetric assays employ identical monoclonal antibodies for capture and detection of Salmonella as used in the fluorescent assay. In this comparative study, the performance of each new assay was consistent with the performance of method 989.15. These methods have been adopted official first action by AOAC as alternative methods for the detection of Salmonella in foods.
M S Curiale; M J Klatt; W D Gehle; H E Chandonnet
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal - Association of Official Analytical Chemists     Volume:  73     ISSN:  0004-5756     ISO Abbreviation:  J Assoc Off Anal Chem     Publication Date:    1990 Nov-Dec
Date Detail:
Created Date:  1991-04-03     Completed Date:  1991-04-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7505559     Medline TA:  J Assoc Off Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  961-8     Citation Subset:  IM    
Silliker Laboratories, Inc., Chicago Heights, IL 60411.
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MeSH Terms
Food Microbiology*
Immunoenzyme Techniques
Salmonella / isolation & purification*
Sensitivity and Specificity

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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