Document Detail


Colorimetric determination of succinic acid using yeast succinate dehydrogenase.
MedLine Citation:
PMID:  9762145     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An enzymatic method for the rapid determination of succinic acid in biological fluids was developed utilizing yeast mitochondria as a source of succinate dehydrogenase. The yeast enzyme catalyzes a complete stoichiometric reduction of 2- (p-iodophenyl)-3-(p-nitrophenyl)-5-tetrazolium chloride to a red formazan. The formazan is extracted into ethylacetate and its absorbance measured at 490 nm. The method is simple, specific, reproducible, and very sensitive (0.01 to 0.14 mumol). The yeast enzyme can be stored in liquid nitrogen for periods of at least 30 days with no significant change in specific activity. In this respect it is superior to a variety of succinate dehydrogenase preparations from animal tissues. The method was applied to measurement of succinic acid excreted by nonproliferating yeast cells metabolizing glucose. Derepressed yeast cells secreted several-fold as much succinic acid as repressed cells submitted to identical test conditions.
Authors:
A B Valle; A D Panek; J R Mattoon
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  91     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1978 Dec 
Date Detail:
Created Date:  1998-11-06     Completed Date:  1998-11-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  583-99     Citation Subset:  IM    
Affiliation:
Federal University of Rio de Janeiro, Institute of Chemistry, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Colorimetry / methods*
Mitochondria / enzymology
Mitochondria, Liver / enzymology
Rats
Saccharomyces cerevisiae / enzymology
Succinate Dehydrogenase / isolation & purification
Succinic Acid / analysis*
Grant Support
ID/Acronym/Agency:
GM15884/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
110-15-6/Succinic Acid; EC 1.3.99.1/Succinate Dehydrogenase

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