Document Detail


Colorimetric determination of sildenafil citrate (Viagra) through ion-associate complex formation.
MedLine Citation:
PMID:  19382569     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple, quick, accurate, and sensitive colorimetric method is described for the determination of sildenafil citrate (SLD). The method is based on the reaction of SLD with Congo Red, Sudan II, and Gentian Violet in buffered aqueous solutions at pH 2.5, 6.5, and 11.0, respectively, to give highly colored soluble ion-associate complex species; the colored products are quantitated colorimetrically at 523, 554, and 569 nm, respectively. The various experimental conditions were optimized. The stoichiometric ratio was found to be 1:1 for all ion associates; the calculated logarithmic stability constants were 8.51, 7.79, and 5.58, respectively. Beer's law was obeyed over the concentration range of 0.2-7.0 microg/mL, whereas the Ringbom optimum concentration range was 0.4-6.5 microg/mL. Values for molar absorptivity, Sandell sensitivity, and detection and quantification limits were also calculated. The proposed method was successfully applied to the determination of SLD in Viagra tablets and in serum samples by using the technique of standard additions with mean accuracy values of 100.06 +/- 1.14, 99.87 +/- 0.70, and 99.86 +/- 0.97% for Viagra tablets and 99.88 +/- 0.60, 99.90 +/- 0.90, and 100.24 +/- 0.80% for serum samples, respectively.
Authors:
Alaa S Amin; Moustafa E Moustafa; Reham El-Dosoky
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  92     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2009 Jan-Feb
Date Detail:
Created Date:  2009-04-22     Completed Date:  2009-06-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  125-30     Citation Subset:  IM    
Affiliation:
Benha University, Faculty of Science, Chemistry Department, Benha, Egypt. asamin2002@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Colorimetry / methods
Congo Red
Erectile Dysfunction / drug therapy
Gentian Violet
Humans
Indicators and Reagents
Male
Piperazines / analysis*,  blood,  therapeutic use
Purines / analysis,  blood,  therapeutic use
Sensitivity and Specificity
Solutions
Sulfones / analysis*,  blood,  therapeutic use
Temperature
Vasodilator Agents / analysis,  blood
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 0/Piperazines; 0/Purines; 0/Solutions; 0/Sulfones; 0/Vasodilator Agents; 139755-83-2/sildenafil; 548-62-9/Gentian Violet; 573-58-0/Congo Red

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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