Document Detail


Colorimetric detection and spectrophotometric determination of the aminothiazolyl-alkoxyimino beta-lactams.
MedLine Citation:
PMID:  3733516     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The 2-aminothiazolyl-4-yl-2-alkoxyiminoacetamido substituent-containing beta-lactam antibiotics (cephalosporins and monobactams) develop a stable, concentration-dependent purple or cherry-red color after reaction with sodium nitrite in acidic condition. The color-formation is highly specific; it requires certain defined structural features such as the simultaneous presence of the intact aminothiazole-ring and an alkoxyimino substituent in the syn configuration. Other substituents on the beta-lactam nucleus have effect only on the intensity of the color. This simple and fast colorimetric procedure was found to be useful not only for the detection of this class of beta-lactam antibiotics but also for their quantitative spectrophotometric determination (lambda max 500 nm). A linear relationship exists between the intensity of the color plotted on a logarithmic scale and the concentration (12.5-200 micrograms/ml) of the compounds on an arithmetic scale. The beta-lactams studied in this class with definitely positive purple-red color reaction are; cefotaxime, ceftizoxime, ceftazidime, ceftriaxone, cefmenoxime, cefodizime, ceftiolene, cefpirome, aztreonam, HR 109, FK 027 (cefixime), FR 19346, SK&F 88070, FR 13300, carumonam, YM 13115, BMY 28142, DN 9550, deacetylcefotaxime, deacetoxycefotaxime and deacetylcefotaxime lactone.
Authors:
J V Uri; T C Jain
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of antibiotics     Volume:  39     ISSN:  0021-8820     ISO Abbreviation:  J. Antibiot.     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-09-17     Completed Date:  1986-09-17     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  0151115     Medline TA:  J Antibiot (Tokyo)     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  669-75     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / analysis*
Cephalosporins / analysis
Colorimetry
Spectrophotometry
Structure-Activity Relationship
Grant Support
ID/Acronym/Agency:
091625//Wellcome Trust
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Cephalosporins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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