| A colorimetric method for detection of K-ras codon 12 point mutations in DNA extracted from tissue and peripheral blood in pancreatic disorders. | |
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MedLine Citation:
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PMID: 20383741 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Molecular-based methods to monitor point mutations require special and expensive equipment unavailable in most hospitals. Colorimetric-based analysis is an ideal platform for K-ras codon 12 gene point mutations because it uses commonly found hospital equipment. The colorimetric assay is sensitive and specific, detecting mutated DNA levels as low as 1% in a wild-type background. Paired genomic DNA extracts of fixed tissue and cellular fractions of peripheral blood are more sensitive and accurate than unpaired samplings. This approach has the potential to improve K-ras point mutation scans as well as to detect micrometastases in circulating tumor cells. |
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Authors:
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Robert A Ollar; Avram M Cooperman; Michael E Wayne; James F Barrecchia; Niket Sonpal; Sushil Duddempudi; Franklin E Kasmin |
Publication Detail:
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Type: Journal Article Date: 2010-04-11 |
Journal Detail:
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Title: Biochemical genetics Volume: 48 ISSN: 1573-4927 ISO Abbreviation: Biochem. Genet. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2010-10-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0126611 Medline TA: Biochem Genet Country: United States |
Other Details:
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Languages: eng Pagination: 577-89 Citation Subset: IM |
Affiliation:
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Department of Surgery, St. Vincent's Hospital Manhattan, New York, NY 10011, USA. rao48@yahoo.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Codon
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genetics* Colorimetry / methods* DNA / blood*, genetics* Humans Pancreatic Diseases / blood, genetics*, pathology Point Mutation / genetics* Proto-Oncogene Proteins / genetics* ras Proteins / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Codon; 0/KRAS protein, human; 0/Proto-Oncogene Proteins; 9007-49-2/DNA; EC 3.6.5.2/ras Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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