| Colorectal anastomotic leakage: a new experimental model. | |
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MedLine Citation:
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PMID: 19446852 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Anastomotic leakage is the major complication after colorectal surgery. To date, animal experiments concerning colorectal anastomosis focus on anastomotic healing instead of anastomotic leakage. This study aims to develop a new experimental model for colorectal anastomotic leakage. METHODS: A control group, receiving an anastomosis with 12 interrupted sutures, was compared to a group receiving an anastomosis with 6 interrupted sutures. When the leakage rate was observed to be too low, the number of sutures was decreased stepwise, to 5 or less. Each group contained 9 "C57Bl6-mice". After 7 d the Anastomotic Bursting Pressure (ABP) was determined. RESULTS: In the first experiment, one mouse (11.1%) in the case group and none in the control group developed leakage. Average ABP was 152.2 mmHg in the control group and 138,8 mmHg in the case group (P=0.111). In the second experiment, case group receiving an anastomosis with 5 sutures, 4 mice (44.4%) in the case group developed leakage. This experiment was repeated twice resulting in leakage rates of 33.3% and 44.4%. The average overall ABP in the case group was 142.7 mmHg vs. 179.9 mmHg (P=0.022) in the control group. The mice without leakage showed a stabilization of average weight loss around day 2 and 3 and a decrease afterwards. The mice with leakage showed a decrease only after day 5. The difference in wellness-scores between the groups with- and without leakage was 2 points, increasing during follow-up. CONCLUSIONS: The model of anastomotic leakage caused by creating an anastomosis with 5 interrupted sutures is feasible. Weight loss and wellness-scores are good predictors of leakage. |
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Authors:
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Niels Komen; Hans-Christiaan van der Wal; Max Ditzel; Gert-Jan Kleinrensink; Hans Jeekel; Johan F Lange |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-16 |
Journal Detail:
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Title: The Journal of surgical research Volume: 155 ISSN: 1095-8673 ISO Abbreviation: J. Surg. Res. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-06-22 Completed Date: 2009-07-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: United States |
Other Details:
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Languages: eng Pagination: 7-12 Citation Subset: IM |
Affiliation:
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Department of Surgery, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands. nielskomen@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anastomosis, Surgical
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adverse effects* Animals Disease Models, Animal* Intestinal Perforation / etiology* Mice* Mice, Inbred C57BL Suture Techniques |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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