Document Detail

Color-tuned channelrhodopsins for multiwavelength optogenetics.
MedLine Citation:
PMID:  22843694     Owner:  NLM     Status:  MEDLINE    
Channelrhodopsin-2 is a light-gated ion channel and a major tool of optogenetics. It is used to control neuronal activity via blue light. Here we describe the construction of color-tuned high efficiency channelrhodopsins (ChRs), based on chimeras of Chlamydomonas channelrhodopsin-1 and Volvox channelrhodopsin-1. These variants show superb expression and plasma membrane integration, resulting in 3-fold larger photocurrents in HEK cells compared with channelrhodopsin-2. Further molecular engineering gave rise to chimeric variants with absorption maxima ranging from 526 to 545 nm, dovetailing well with maxima of channelrhodopsin-2 derivatives ranging from 461 to 492 nm. Additional kinetic fine-tuning led to derivatives in which the lifetimes of the open state range from 19 ms to 5 s. Finally, combining green- with blue-absorbing variants allowed independent activation of two distinct neural cell populations at 560 and 405 nm. This novel panel of channelrhodopsin variants may serve as an important toolkit element for dual-color cell stimulation in neural circuits.
Matthias Prigge; Franziska Schneider; Satoshi P Tsunoda; Carrie Shilyansky; Jonas Wietek; Karl Deisseroth; Peter Hegemann
Related Documents :
22821724 - Switchable catalysis.
11536734 - Infrared light-emitting diode radiation causes gravitropic and morphological effects in...
282834 - The effect of irradiation and heat on the content of adrenaline in commercially manufac...
16660684 - Effect of red light on geotropism in pea epicotyls.
9153114 - Neurobehavioral studies of the central auditory system.
17114404 - Cuttlefish responses to visual orientation of substrates, water flow and a model of mot...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-27
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  287     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-17     Completed Date:  2012-12-04     Revised Date:  2013-09-17    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  31804-12     Citation Subset:  IM    
Institute of Biology, Experimental Biophysics, Humboldt-Universität zu Berlin, 10115 Berlin, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Calcium / chemistry,  metabolism
Chlamydomonas / metabolism*
Electrophysiology / methods
Genetic Engineering / methods
HEK293 Cells
Hippocampus / metabolism
Models, Neurological
Oocytes / metabolism
Optogenetics / methods*
Recombinant Fusion Proteins / chemistry,  metabolism
Rhodopsin / chemistry*
Volvox / metabolism*
Grant Support
//Howard Hughes Medical Institute
Reg. No./Substance:
0/Ions; 0/Recombinant Fusion Proteins; 7440-70-2/Calcium; 9009-81-8/Rhodopsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Tetraspanin CD151 stimulates adhesion-dependent activation of Ras, Rac, and Cdc42 by facilitating mo...
Next Document:  Novel one-step immunoassays to quantify ?-synuclein: applications for biomarker development and high...