Document Detail


Color-tuned channelrhodopsins for multiwavelength optogenetics.
MedLine Citation:
PMID:  22843694     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Channelrhodopsin-2 is a light-gated ion channel and a major tool of optogenetics. It is used to control neuronal activity via blue light. Here we describe the construction of color-tuned high efficiency channelrhodopsins (ChRs), based on chimeras of Chlamydomonas channelrhodopsin-1 and Volvox channelrhodopsin-1. These variants show superb expression and plasma membrane integration, resulting in 3-fold larger photocurrents in HEK cells compared with channelrhodopsin-2. Further molecular engineering gave rise to chimeric variants with absorption maxima ranging from 526 to 545 nm, dovetailing well with maxima of channelrhodopsin-2 derivatives ranging from 461 to 492 nm. Additional kinetic fine-tuning led to derivatives in which the lifetimes of the open state range from 19 ms to 5 s. Finally, combining green- with blue-absorbing variants allowed independent activation of two distinct neural cell populations at 560 and 405 nm. This novel panel of channelrhodopsin variants may serve as an important toolkit element for dual-color cell stimulation in neural circuits.
Authors:
Matthias Prigge; Franziska Schneider; Satoshi P Tsunoda; Carrie Shilyansky; Jonas Wietek; Karl Deisseroth; Peter Hegemann
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-27
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  287     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-17     Completed Date:  2012-12-04     Revised Date:  2013-09-17    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  31804-12     Citation Subset:  IM    
Affiliation:
Institute of Biology, Experimental Biophysics, Humboldt-Universität zu Berlin, 10115 Berlin, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / chemistry,  metabolism
Chlamydomonas / metabolism*
Color
Electrophysiology / methods
Genetic Engineering / methods
HEK293 Cells
Hippocampus / metabolism
Humans
Ions
Kinetics
Light
Models, Neurological
Oocytes / metabolism
Optogenetics / methods*
Recombinant Fusion Proteins / chemistry,  metabolism
Rhodopsin / chemistry*
Volvox / metabolism*
Xenopus
Grant Support
ID/Acronym/Agency:
//Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Ions; 0/Recombinant Fusion Proteins; 7440-70-2/Calcium; 9009-81-8/Rhodopsin
Comments/Corrections

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