| Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food. | |
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MedLine Citation:
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PMID: 20579482 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Food contamination caused by chemical hazards such as persistent organic pollutants (POPs) is a worldwide public health concern and requires continuous monitoring. The chromatography-based analysis methods for POPs are accurate and quite sensitive but they are time-consuming, laborious and expensive. Thus, there is a need for validated simplified screening tools, which are inexpensive, rapid, have automation potential and can detect multiple POPs simultaneously. In this study we developed a flow cytometry-based immunoassay (FCIA) using a color-encoded microbeads technology to detect benzo[a]pyrene (BaP) and other polycyclic aromatic hydrocarbons (PAHs) in buffer and food extracts as a starting point for the future development of rapid multiplex assays including other POPs in food, such as polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs). A highly sensitive assay for BaP was obtained with an IC(50) of 0.3 microg L(-1) using a monoclonal antibody (Mab22F12) against BaP, similar to the IC(50) of a previously described enzyme-linked immunosorbent assay (ELISA) using the same Mab. Moreover, the FCIA was 8 times more sensitive for BaP compared to a surface plasmon resonance (SPR)-based biosensor immunoassay (BIA) using the same reagents. The selectivity of the FCIAs was tested, with two Mabs against BaP for 25 other PAHs, including two hydroxyl PAH metabolites. Apart from BaP, the FCIAs can detect PAHs such as indenol[1,2,3-cd]pyrene (IP), benz[a]anthracene (BaA), and chrysene (CHR) which are also appointed by the European Food Safety Authority (EFSA) as suitable indicators of PAH contamination in food. The FCIAs results were in agreement with those obtained with gas chromatography-mass spectrometry (GC-MS) for the detection of PAHs in real food samples of smoked carp and wheat flour and has great potential for the future routine application of this assay in a simplex or multiplex format in combination with simplified extraction procedure which are under development. |
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Authors:
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Anastasia Meimaridou; Willem Haasnoot; Linda Noteboom; Dimitrios Mintzas; Jana Pulkrabova; Jana Hajslová; Michel W F Nielen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-10 |
Journal Detail:
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Title: Analytica chimica acta Volume: 672 ISSN: 1873-4324 ISO Abbreviation: Anal. Chim. Acta Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-28 Completed Date: 2010-09-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370534 Medline TA: Anal Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 9-14 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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RIKILT-Institute of Food Safety, Wageningen UR, P.O. Box 230, 6700 AE Wageningen, The Netherlands. anastasia.meimaridou@wur.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / immunology Benzo(a)pyrene / analysis Carps Color Fish Products / analysis Flour / analysis Flow Cytometry / methods* Food Contamination / analysis* Immunoassay Microspheres Polycyclic Hydrocarbons, Aromatic / analysis*, immunology Sensitivity and Specificity Triticum / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Polycyclic Hydrocarbons, Aromatic; 50-32-8/Benzo(a)pyrene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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