| Color compensation of multicolor fish images. | |
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MedLine Citation:
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PMID: 19116195 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Multicolor fluorescence in situ hybridization (M-FISH) techniques provide color karyotyping that allows simultaneous analysis of numerical and structural abnormalities of whole human chromosomes. Chromosomes are stained combinatorially in M-FISH. By analyzing the intensity combinations of each pixel, all chromosome pixels in an image are classified. Due to the overlap of excitation and emission spectra and the broad sensitivity of image sensors, the obtained images contain crosstalk between the color channels. The crosstalk complicates both visual and automatic image analysis and may eventually affect the classification accuracy in M-FISH. The removal of crosstalk is possible by finding the color compensation matrix, which quantifies the color spillover between channels. However, there exists no simple method of finding the color compensation matrix from multichannel fluorescence images whose specimens are combinatorially hybridized. In this paper, we present a method of calculating the color compensation matrix for multichannel fluorescence images whose specimens are combinatorially stained. |
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Authors:
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Hyohoon Choi; Kenneth R Castleman; Alan C Bovik |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: IEEE transactions on medical imaging Volume: 28 ISSN: 1558-0062 ISO Abbreviation: IEEE Trans Med Imaging Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-31 Completed Date: 2009-03-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8310780 Medline TA: IEEE Trans Med Imaging Country: United States |
Other Details:
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Languages: eng Pagination: 129-36 Citation Subset: IM |
Affiliation:
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Sealed Air Corporation, San Jose, CA 95110 USA. hyohoon@alumni.utexas.net |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Artifacts* Chromosomes, Human / genetics, ultrastructure Color Fluorescence Fluorescent Dyes Humans Microscopy, Fluorescence / methods Sensitivity and Specificity Spectral Karyotyping / methods* Subtraction Technique* |
| Grant Support | |
ID/Acronym/Agency:
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R44 HD-038151/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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